Articles producció científica> Medicina i Cirurgia

Smoking Suppresses the Therapeutic Potential of Adipose Stem Cells in Crohn’s Disease Patients through Epigenetic Changes

  • Datos identificativos

    Identificador: imarina:9295693
    Autores:
    Boronat-Toscano, AVañó, IMonfort-Ferré, DMenacho, MValldosera, GCaro, AEspina, BMañas, MJMarti, MEspin, ESaera-Vila, ASerena, C
    Resumen:
    Patients with Crohn’s disease (CD) who smoke are known to have a worse prognosis than never-smokers and a higher risk for post-surgical recurrence, whereas patients who quit smoking after surgery have significantly lower post-operative recurrence. The hypothesis was that smoking induces epigenetic changes that impair the capacity of adipose stem cells (ASCs) to suppress the immune system. It was also questioned whether this impairment remains in ex-smokers with CD. ASCs were isolated from non-smokers, smokers and ex-smokers with CD and their interactions with immune cells were studied. The ASCs from both smokers and ex-smokers promoted macrophage polarization to an M1 pro-inflammatory phenotype, were not able to inhibit T- and B-cell proliferation in vitro and enhanced the gene and protein expression of inflammatory markers including interleukin-1b. Genome-wide epigenetic analysis using two different bioinformatic approaches revealed significant changes in the methylation patterns of genes that are critical for wound healing, immune and metabolic response and p53-mediated DNA damage response in ASCs from smokers and ex-smokers with CD. In conclusion, cigarette smoking induces a pro-inflammatory epigenetic signature in ASCs that likely compromises their therapeutic potential.
  • Otros:

    Autor según el artículo: Boronat-Toscano, A; Vañó, I; Monfort-Ferré, D; Menacho, M; Valldosera, G; Caro, A; Espina, B; Mañas, MJ; Marti, M; Espin, E; Saera-Vila, A; Serena, C
    Departamento: Medicina i Cirurgia
    Autor/es de la URV: Boronat Toscano, Albert / Menacho Viladot, Margarita / Monfort Ferre, Diandra / Serena Perelló, Carolina
    Palabras clave: Tobacco Macrophages Inflammatory bowel diseases Immune cells Dna methylation Differentially methylated regions Cigarette Chronic inflammatory disease Cell therapy Adipose tissue tobacco macrophages inflammatory bowel diseases immune cells differentially methylated regions cigarette chronic inflammatory disease cell therapy adipose tissue
    Resumen: Patients with Crohn’s disease (CD) who smoke are known to have a worse prognosis than never-smokers and a higher risk for post-surgical recurrence, whereas patients who quit smoking after surgery have significantly lower post-operative recurrence. The hypothesis was that smoking induces epigenetic changes that impair the capacity of adipose stem cells (ASCs) to suppress the immune system. It was also questioned whether this impairment remains in ex-smokers with CD. ASCs were isolated from non-smokers, smokers and ex-smokers with CD and their interactions with immune cells were studied. The ASCs from both smokers and ex-smokers promoted macrophage polarization to an M1 pro-inflammatory phenotype, were not able to inhibit T- and B-cell proliferation in vitro and enhanced the gene and protein expression of inflammatory markers including interleukin-1b. Genome-wide epigenetic analysis using two different bioinformatic approaches revealed significant changes in the methylation patterns of genes that are critical for wound healing, immune and metabolic response and p53-mediated DNA damage response in ASCs from smokers and ex-smokers with CD. In conclusion, cigarette smoking induces a pro-inflammatory epigenetic signature in ASCs that likely compromises their therapeutic potential.
    Áreas temáticas: Medicine (miscellaneous) Cell biology Biochemistry, genetics and molecular biology (miscellaneous) Biochemistry, genetics and molecular biology (all)
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: margarita.menacho@urv.cat carolina.serena@urv.cat albert.boronat@estudiants.urv.cat diandra.monfort@estudiants.urv.cat
    Identificador del autor: 0000-0003-3832-4249
    Fecha de alta del registro: 2024-08-03
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://www.mdpi.com/2073-4409/12/7/1021
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Cells. 12 (7): 1021-
    Referencia de l'ítem segons les normes APA: Boronat-Toscano, A; Vañó, I; Monfort-Ferré, D; Menacho, M; Valldosera, G; Caro, A; Espina, B; Mañas, MJ; Marti, M; Espin, E; Saera-Vila, A; Serena, C (2023). Smoking Suppresses the Therapeutic Potential of Adipose Stem Cells in Crohn’s Disease Patients through Epigenetic Changes. Cells, 12(7), 1021-. DOI: 10.3390/cells12071021
    DOI del artículo: 10.3390/cells12071021
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2023
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Biochemistry, Genetics and Molecular Biology (Miscellaneous),Cell Biology,Medicine (Miscellaneous)
    Tobacco
    Macrophages
    Inflammatory bowel diseases
    Immune cells
    Dna methylation
    Differentially methylated regions
    Cigarette
    Chronic inflammatory disease
    Cell therapy
    Adipose tissue
    tobacco
    macrophages
    inflammatory bowel diseases
    immune cells
    differentially methylated regions
    cigarette
    chronic inflammatory disease
    cell therapy
    adipose tissue
    Medicine (miscellaneous)
    Cell biology
    Biochemistry, genetics and molecular biology (miscellaneous)
    Biochemistry, genetics and molecular biology (all)
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