Autor segons l'article: Rodriguez-Muguruza, Samantha; Altuna-Coy, Antonio; Arreaza-Gil, Veronica; Mendieta-Homs, Marina; Castro-Oreiro, Sonia; Poveda-Elices, Maria Jose; del Castillo-Pinol, Nuria; Fontova-Garrofe, Ramon; Chacon, Matilde R
Departament: Bioquímica i Biotecnologia
Autor/s de la URV: Arreaza Gil, Verónica
Paraules clau: Arthritis, rheumatoid Biomarkers Biomarkers 2 Diagnostic Early rheumatoid arthritis Glyceric acid Glyceric acids Humans Lactic acid Metabolomics Serum
Resum: ObjectiveThere is an urgent need for novel biomarkers to improve the early diagnosis of rheumatoid arthritis (ERA). Current serum biomarkers used in the management of ERA, including rheumatoid factor and anti-cyclic citrullinated peptide (ACPA), show limited specificity and sensitivity. Here, we used metabolomics to uncover new serum biomarkers of ERA.MethodsWe applied an untargeted metabolomics approach including gas chromatography time-of-flight mass spectrometry in serum samples from an ERA cohort (n=32) and healthy controls (n=19). Metabolite set enrichment analysis was performed to explore potentially important biological pathways. Partial least squares discriminant analysis and variable importance in projection analysis were performed to construct an ERA biomarker panel.ResultsSignificant differences in the content of 11/81 serum metabolites were identified in patients with ERA. Receiver operating characteristic (ROC) analysis showed that a panel of only three metabolites (glyceric acid, lactic acid, and 3-hydroxisovaleric acid) could correctly classify 96.7% of patients with ERA, with an area under the ROC curve of 0.963 and with 94.4% specificity and 93.5% sensitivity, outperforming ACPA-based diagnosis by 2.9% and, thus, improving the preclinical detection of ERA. Aminoacyl-tRNA biosynthesis and serine, glycine, and phenylalanine metabolism were the most significant dysregulated pathways in patients with ERA.ConclusionA metabolomics serum-based biomarker panel composed of glyceric acid, lactic acid, and 3-hydroxisovaleric acid offers potential for the early clinical diagnosis of RA.
Àrees temàtiques: Biodiversidade Biotecnología Ciência de alimentos Ciências biológicas i Ciências biológicas ii Ciências biológicas iii Engenharias ii Engenharias iii Farmacia Immunology Immunology and allergy Interdisciplinar Medicina i Medicina ii Medicina iii Medicina veterinaria Nutrição Odontología Química Saúde coletiva Zootecnia / recursos pesqueiros
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: veronica.arreaza@urv.cat veronica.arreaza@urv.cat veronica.arreaza@urv.cat
Identificador de l'autor: 0000-0002-8301-2095 0000-0002-8301-2095 0000-0002-8301-2095
Data d'alta del registre: 2024-10-12
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1253913/full
Referència a l'article segons font original: Frontiers In Immunology. 14 1253913-
Referència de l'ítem segons les normes APA: Rodriguez-Muguruza, Samantha; Altuna-Coy, Antonio; Arreaza-Gil, Veronica; Mendieta-Homs, Marina; Castro-Oreiro, Sonia; Poveda-Elices, Maria Jose; del (2023). A serum metabolic biomarker panel for early rheumatoid arthritis. Frontiers In Immunology, 14(), 1253913-. DOI: 10.3389/fimmu.2023.1253913
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI de l'article: 10.3389/fimmu.2023.1253913
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2023
Tipus de publicació: Journal Publications