Articles producció científica> Bioquímica i Biotecnologia

A serum metabolic biomarker panel for early rheumatoid arthritis

  • Dades identificatives

    Identificador: imarina:9384822
    Autors:
    Rodriguez-Muguruza, SamanthaAltuna-Coy, AntonioArreaza-Gil, VeronicaMendieta-Homs, MarinaCastro-Oreiro, SoniaPoveda-Elices, Maria Josedel Castillo-Pinol, NuriaFontova-Garrofe, RamonChacon, Matilde R
    Resum:
    ObjectiveThere is an urgent need for novel biomarkers to improve the early diagnosis of rheumatoid arthritis (ERA). Current serum biomarkers used in the management of ERA, including rheumatoid factor and anti-cyclic citrullinated peptide (ACPA), show limited specificity and sensitivity. Here, we used metabolomics to uncover new serum biomarkers of ERA.MethodsWe applied an untargeted metabolomics approach including gas chromatography time-of-flight mass spectrometry in serum samples from an ERA cohort (n=32) and healthy controls (n=19). Metabolite set enrichment analysis was performed to explore potentially important biological pathways. Partial least squares discriminant analysis and variable importance in projection analysis were performed to construct an ERA biomarker panel.ResultsSignificant differences in the content of 11/81 serum metabolites were identified in patients with ERA. Receiver operating characteristic (ROC) analysis showed that a panel of only three metabolites (glyceric acid, lactic acid, and 3-hydroxisovaleric acid) could correctly classify 96.7% of patients with ERA, with an area under the ROC curve of 0.963 and with 94.4% specificity and 93.5% sensitivity, outperforming ACPA-based diagnosis by 2.9% and, thus, improving the preclinical detection of ERA. Aminoacyl-tRNA biosynthesis and serine, glycine, and phenylalanine metabolism were the most significant dysregulated pathways in patients with ERA.ConclusionA metabolomics serum-based biomarker panel composed of glyceric acid, lactic acid, and 3-hydroxisovaleric acid offers potential for the early clinical diagnosis of RA.
  • Altres:

    Autor segons l'article: Rodriguez-Muguruza, Samantha; Altuna-Coy, Antonio; Arreaza-Gil, Veronica; Mendieta-Homs, Marina; Castro-Oreiro, Sonia; Poveda-Elices, Maria Jose; del Castillo-Pinol, Nuria; Fontova-Garrofe, Ramon; Chacon, Matilde R
    Departament: Bioquímica i Biotecnologia
    Autor/s de la URV: Arreaza Gil, Verónica
    Paraules clau: Arthritis, rheumatoid Biomarkers Biomarkers 2 Diagnostic Early rheumatoid arthritis Glyceric acid Glyceric acids Humans Lactic acid Metabolomics Serum
    Resum: ObjectiveThere is an urgent need for novel biomarkers to improve the early diagnosis of rheumatoid arthritis (ERA). Current serum biomarkers used in the management of ERA, including rheumatoid factor and anti-cyclic citrullinated peptide (ACPA), show limited specificity and sensitivity. Here, we used metabolomics to uncover new serum biomarkers of ERA.MethodsWe applied an untargeted metabolomics approach including gas chromatography time-of-flight mass spectrometry in serum samples from an ERA cohort (n=32) and healthy controls (n=19). Metabolite set enrichment analysis was performed to explore potentially important biological pathways. Partial least squares discriminant analysis and variable importance in projection analysis were performed to construct an ERA biomarker panel.ResultsSignificant differences in the content of 11/81 serum metabolites were identified in patients with ERA. Receiver operating characteristic (ROC) analysis showed that a panel of only three metabolites (glyceric acid, lactic acid, and 3-hydroxisovaleric acid) could correctly classify 96.7% of patients with ERA, with an area under the ROC curve of 0.963 and with 94.4% specificity and 93.5% sensitivity, outperforming ACPA-based diagnosis by 2.9% and, thus, improving the preclinical detection of ERA. Aminoacyl-tRNA biosynthesis and serine, glycine, and phenylalanine metabolism were the most significant dysregulated pathways in patients with ERA.ConclusionA metabolomics serum-based biomarker panel composed of glyceric acid, lactic acid, and 3-hydroxisovaleric acid offers potential for the early clinical diagnosis of RA.
    Àrees temàtiques: Biodiversidade Biotecnología Ciência de alimentos Ciências biológicas i Ciências biológicas ii Ciências biológicas iii Engenharias ii Engenharias iii Farmacia Immunology Immunology and allergy Interdisciplinar Medicina i Medicina ii Medicina iii Medicina veterinaria Nutrição Odontología Química Saúde coletiva Zootecnia / recursos pesqueiros
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    Adreça de correu electrònic de l'autor: veronica.arreaza@urv.cat veronica.arreaza@urv.cat veronica.arreaza@urv.cat
    Identificador de l'autor: 0000-0002-8301-2095 0000-0002-8301-2095 0000-0002-8301-2095
    Data d'alta del registre: 2024-10-12
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1253913/full
    Referència a l'article segons font original: Frontiers In Immunology. 14 1253913-
    Referència de l'ítem segons les normes APA: Rodriguez-Muguruza, Samantha; Altuna-Coy, Antonio; Arreaza-Gil, Veronica; Mendieta-Homs, Marina; Castro-Oreiro, Sonia; Poveda-Elices, Maria Jose; del (2023). A serum metabolic biomarker panel for early rheumatoid arthritis. Frontiers In Immunology, 14(), 1253913-. DOI: 10.3389/fimmu.2023.1253913
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI de l'article: 10.3389/fimmu.2023.1253913
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2023
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Immunology,Immunology and Allergy
    Arthritis, rheumatoid
    Biomarkers
    Biomarkers 2
    Diagnostic
    Early rheumatoid arthritis
    Glyceric acid
    Glyceric acids
    Humans
    Lactic acid
    Metabolomics
    Serum
    Biodiversidade
    Biotecnología
    Ciência de alimentos
    Ciências biológicas i
    Ciências biológicas ii
    Ciências biológicas iii
    Engenharias ii
    Engenharias iii
    Farmacia
    Immunology
    Immunology and allergy
    Interdisciplinar
    Medicina i
    Medicina ii
    Medicina iii
    Medicina veterinaria
    Nutrição
    Odontología
    Química
    Saúde coletiva
    Zootecnia / recursos pesqueiros
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