Autor según el artículo: Rodriguez-Muguruza, Samantha; Altuna-Coy, Antonio; Arreaza-Gil, Veronica; Mendieta-Homs, Marina; Castro-Oreiro, Sonia; Poveda-Elices, Maria Jose; del Castillo-Pinol, Nuria; Fontova-Garrofe, Ramon; Chacon, Matilde R
Departamento: Bioquímica i Biotecnologia
Autor/es de la URV: Arreaza Gil, Verónica
Palabras clave: Arthritis, rheumatoid Biomarkers Biomarkers 2 Diagnostic Early rheumatoid arthritis Glyceric acid Glyceric acids Humans Lactic acid Metabolomics Serum
Resumen: ObjectiveThere is an urgent need for novel biomarkers to improve the early diagnosis of rheumatoid arthritis (ERA). Current serum biomarkers used in the management of ERA, including rheumatoid factor and anti-cyclic citrullinated peptide (ACPA), show limited specificity and sensitivity. Here, we used metabolomics to uncover new serum biomarkers of ERA.MethodsWe applied an untargeted metabolomics approach including gas chromatography time-of-flight mass spectrometry in serum samples from an ERA cohort (n=32) and healthy controls (n=19). Metabolite set enrichment analysis was performed to explore potentially important biological pathways. Partial least squares discriminant analysis and variable importance in projection analysis were performed to construct an ERA biomarker panel.ResultsSignificant differences in the content of 11/81 serum metabolites were identified in patients with ERA. Receiver operating characteristic (ROC) analysis showed that a panel of only three metabolites (glyceric acid, lactic acid, and 3-hydroxisovaleric acid) could correctly classify 96.7% of patients with ERA, with an area under the ROC curve of 0.963 and with 94.4% specificity and 93.5% sensitivity, outperforming ACPA-based diagnosis by 2.9% and, thus, improving the preclinical detection of ERA. Aminoacyl-tRNA biosynthesis and serine, glycine, and phenylalanine metabolism were the most significant dysregulated pathways in patients with ERA.ConclusionA metabolomics serum-based biomarker panel composed of glyceric acid, lactic acid, and 3-hydroxisovaleric acid offers potential for the early clinical diagnosis of RA.
Áreas temáticas: Biodiversidade Biotecnología Ciência de alimentos Ciências biológicas i Ciências biológicas ii Ciências biológicas iii Engenharias ii Engenharias iii Farmacia Immunology Immunology and allergy Interdisciplinar Medicina i Medicina ii Medicina iii Medicina veterinaria Nutrição Odontología Química Saúde coletiva Zootecnia / recursos pesqueiros
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: veronica.arreaza@urv.cat veronica.arreaza@urv.cat veronica.arreaza@urv.cat
Identificador del autor: 0000-0002-8301-2095 0000-0002-8301-2095 0000-0002-8301-2095
Fecha de alta del registro: 2024-10-12
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1253913/full
Referencia al articulo segun fuente origial: Frontiers In Immunology. 14 1253913-
Referencia de l'ítem segons les normes APA: Rodriguez-Muguruza, Samantha; Altuna-Coy, Antonio; Arreaza-Gil, Veronica; Mendieta-Homs, Marina; Castro-Oreiro, Sonia; Poveda-Elices, Maria Jose; del (2023). A serum metabolic biomarker panel for early rheumatoid arthritis. Frontiers In Immunology, 14(), 1253913-. DOI: 10.3389/fimmu.2023.1253913
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI del artículo: 10.3389/fimmu.2023.1253913
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2023
Tipo de publicación: Journal Publications