Articles producció científica> Psicologia

ProAKAP4 as a motility long-lasting marker in Catalan donkey spermatozoa

  • Dades identificatives

    Identificador: imarina:9385426
    Autors:
    Dordas-Perpinya, MartaSergeant, NicolasYanez-Ortiz, IvanMevel, VincentCatalan, JaimeBruyas, Jean-FrancoisBriand-Amirat, LamiaMiro, Jordi
    Resum:
    ProAKAP4 is identified within the flagellum of spermatozoa in various mammalian species, serving as a structural protein associated with motility parameters. This investigation focuses on the presence of proAKAP4 in donkey sperm, elucidating its localization, molecular characteristics, and its correlation with motility descriptors and mitochondrial membrane potential. Twelve ejaculates from Catalan donkeys were analyzed in this study. The initial steps involved proAKAP4 sequencing and detection through Western blotting and immunofluorescence. Post-thaw assessments were conducted at 0, 1, and 3 h, encompassing proAKAP4 levels, sperm motility analyzed via Computer-Assisted Sperm Analysis (CASA), and mitochondrial membrane potential determined by flow cytometry using the JC-1 stain. The findings reveal that proAKAP4 in donkeys exhibits a characteristic localization at the principal piece of the flagellum, consistent with observations in other mammals. The molecular weight of proAKAP4 is determined to be 100 kDa. Significantly, a positive correlation (p
  • Altres:

    Autor segons l'article: Dordas-Perpinya, Marta; Sergeant, Nicolas; Yanez-Ortiz, Ivan; Mevel, Vincent; Catalan, Jaime; Bruyas, Jean-Francois; Briand-Amirat, Lamia; Miro, Jordi
    Departament: Psicologia
    Autor/s de la URV: Miró Martínez, Jordi
    Paraules clau: Akap4 Anchor protein Boa Cryopreservation Donkey Mitochondrial activit Motility Proakap4 Semen Sperm Spermatozoa
    Resum: ProAKAP4 is identified within the flagellum of spermatozoa in various mammalian species, serving as a structural protein associated with motility parameters. This investigation focuses on the presence of proAKAP4 in donkey sperm, elucidating its localization, molecular characteristics, and its correlation with motility descriptors and mitochondrial membrane potential. Twelve ejaculates from Catalan donkeys were analyzed in this study. The initial steps involved proAKAP4 sequencing and detection through Western blotting and immunofluorescence. Post-thaw assessments were conducted at 0, 1, and 3 h, encompassing proAKAP4 levels, sperm motility analyzed via Computer-Assisted Sperm Analysis (CASA), and mitochondrial membrane potential determined by flow cytometry using the JC-1 stain. The findings reveal that proAKAP4 in donkeys exhibits a characteristic localization at the principal piece of the flagellum, consistent with observations in other mammals. The molecular weight of proAKAP4 is determined to be 100 kDa. Significantly, a positive correlation (p
    Àrees temàtiques: Agriculture, dairy & animal science Animal science and zoology Biodiversidade Biotecnología Ciências agrárias i Ciências ambientais Ciências biológicas i Ciências biológicas ii Ciências biológicas iii Economia Endocrinology Farmacia Food animals Geografía Interdisciplinar Materiais Medicina i Medicina ii Medicina iii Medicina veterinaria Medicine (miscellaneous) Nutrição Odontología Química Reproductive biology Veterinary sciences Zootecnia / recursos pesqueiros
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    Adreça de correu electrònic de l'autor: jordi.miro@urv.cat
    Identificador de l'autor: 0000-0002-1998-6653
    Data d'alta del registre: 2024-10-12
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://www.sciencedirect.com/science/article/pii/S0378432024000186?via%3Dihub
    Referència a l'article segons font original: Animal Reproduction Science. 262 107427-
    Referència de l'ítem segons les normes APA: Dordas-Perpinya, Marta; Sergeant, Nicolas; Yanez-Ortiz, Ivan; Mevel, Vincent; Catalan, Jaime; Bruyas, Jean-Francois; Briand-Amirat, Lamia; Miro, Jordi (2024). ProAKAP4 as a motility long-lasting marker in Catalan donkey spermatozoa. Animal Reproduction Science, 262(), 107427-. DOI: 10.1016/j.anireprosci.2024.107427
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI de l'article: 10.1016/j.anireprosci.2024.107427
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2024
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Agriculture, Dairy & Animal Science,Animal Science and Zoology,Endocrinology,Food Animals,Medicine (Miscellaneous),Reproductive Biology,Veterinary Sciences
    Akap4
    Anchor protein
    Boa
    Cryopreservation
    Donkey
    Mitochondrial activit
    Motility
    Proakap4
    Semen
    Sperm
    Spermatozoa
    Agriculture, dairy & animal science
    Animal science and zoology
    Biodiversidade
    Biotecnología
    Ciências agrárias i
    Ciências ambientais
    Ciências biológicas i
    Ciências biológicas ii
    Ciências biológicas iii
    Economia
    Endocrinology
    Farmacia
    Food animals
    Geografía
    Interdisciplinar
    Materiais
    Medicina i
    Medicina ii
    Medicina iii
    Medicina veterinaria
    Medicine (miscellaneous)
    Nutrição
    Odontología
    Química
    Reproductive biology
    Veterinary sciences
    Zootecnia / recursos pesqueiros
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