Autor segons l'article: Dordas-Perpinya, Marta; Sergeant, Nicolas; Yanez-Ortiz, Ivan; Mevel, Vincent; Catalan, Jaime; Bruyas, Jean-Francois; Briand-Amirat, Lamia; Miro, Jordi
Departament: Psicologia
Autor/s de la URV: Miró Martínez, Jordi
Paraules clau: Akap4 Anchor protein Boa Cryopreservation Donkey Mitochondrial activit Motility Proakap4 Semen Sperm Spermatozoa
Resum: ProAKAP4 is identified within the flagellum of spermatozoa in various mammalian species, serving as a structural protein associated with motility parameters. This investigation focuses on the presence of proAKAP4 in donkey sperm, elucidating its localization, molecular characteristics, and its correlation with motility descriptors and mitochondrial membrane potential. Twelve ejaculates from Catalan donkeys were analyzed in this study. The initial steps involved proAKAP4 sequencing and detection through Western blotting and immunofluorescence. Post-thaw assessments were conducted at 0, 1, and 3 h, encompassing proAKAP4 levels, sperm motility analyzed via Computer-Assisted Sperm Analysis (CASA), and mitochondrial membrane potential determined by flow cytometry using the JC-1 stain. The findings reveal that proAKAP4 in donkeys exhibits a characteristic localization at the principal piece of the flagellum, consistent with observations in other mammals. The molecular weight of proAKAP4 is determined to be 100 kDa. Significantly, a positive correlation (p
Àrees temàtiques: Agriculture, dairy & animal science Animal science and zoology Biodiversidade Biotecnología Ciências agrárias i Ciências ambientais Ciências biológicas i Ciências biológicas ii Ciências biológicas iii Economia Endocrinology Farmacia Food animals Geografía Interdisciplinar Materiais Medicina i Medicina ii Medicina iii Medicina veterinaria Medicine (miscellaneous) Nutrição Odontología Química Reproductive biology Veterinary sciences Zootecnia / recursos pesqueiros
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: jordi.miro@urv.cat
Identificador de l'autor: 0000-0002-1998-6653
Data d'alta del registre: 2024-10-12
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Referència a l'article segons font original: Animal Reproduction Science. 262 107427-
Referència de l'ítem segons les normes APA: Dordas-Perpinya, Marta; Sergeant, Nicolas; Yanez-Ortiz, Ivan; Mevel, Vincent; Catalan, Jaime; Bruyas, Jean-Francois; Briand-Amirat, Lamia; Miro, Jordi (2024). ProAKAP4 as a motility long-lasting marker in Catalan donkey spermatozoa. Animal Reproduction Science, 262(), 107427-. DOI: 10.1016/j.anireprosci.2024.107427
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2024
Tipus de publicació: Journal Publications
Repositori URV