Autor según el artículo: Dordas-Perpinya, Marta; Sergeant, Nicolas; Yanez-Ortiz, Ivan; Mevel, Vincent; Catalan, Jaime; Bruyas, Jean-Francois; Briand-Amirat, Lamia; Miro, Jordi
Departamento: Psicologia
Autor/es de la URV: Miró Martínez, Jordi
Palabras clave: Akap4 Anchor protein Boa Cryopreservation Donkey Mitochondrial activit Motility Proakap4 Semen Sperm Spermatozoa
Resumen: ProAKAP4 is identified within the flagellum of spermatozoa in various mammalian species, serving as a structural protein associated with motility parameters. This investigation focuses on the presence of proAKAP4 in donkey sperm, elucidating its localization, molecular characteristics, and its correlation with motility descriptors and mitochondrial membrane potential. Twelve ejaculates from Catalan donkeys were analyzed in this study. The initial steps involved proAKAP4 sequencing and detection through Western blotting and immunofluorescence. Post-thaw assessments were conducted at 0, 1, and 3 h, encompassing proAKAP4 levels, sperm motility analyzed via Computer-Assisted Sperm Analysis (CASA), and mitochondrial membrane potential determined by flow cytometry using the JC-1 stain. The findings reveal that proAKAP4 in donkeys exhibits a characteristic localization at the principal piece of the flagellum, consistent with observations in other mammals. The molecular weight of proAKAP4 is determined to be 100 kDa. Significantly, a positive correlation (p
Áreas temáticas: Agriculture, dairy & animal science Animal science and zoology Biodiversidade Biotecnología Ciências agrárias i Ciências ambientais Ciências biológicas i Ciências biológicas ii Ciências biológicas iii Economia Endocrinology Farmacia Food animals Geografía Interdisciplinar Materiais Medicina i Medicina ii Medicina iii Medicina veterinaria Medicine (miscellaneous) Nutrição Odontología Química Reproductive biology Veterinary sciences Zootecnia / recursos pesqueiros
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: jordi.miro@urv.cat
Identificador del autor: 0000-0002-1998-6653
Fecha de alta del registro: 2024-10-12
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://www.sciencedirect.com/science/article/pii/S0378432024000186?via%3Dihub
Referencia al articulo segun fuente origial: Animal Reproduction Science. 262 107427-
Referencia de l'ítem segons les normes APA: Dordas-Perpinya, Marta; Sergeant, Nicolas; Yanez-Ortiz, Ivan; Mevel, Vincent; Catalan, Jaime; Bruyas, Jean-Francois; Briand-Amirat, Lamia; Miro, Jordi (2024). ProAKAP4 as a motility long-lasting marker in Catalan donkey spermatozoa. Animal Reproduction Science, 262(), 107427-. DOI: 10.1016/j.anireprosci.2024.107427
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI del artículo: 10.1016/j.anireprosci.2024.107427
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2024
Tipo de publicación: Journal Publications
Repositori URV