Articles producció científicaMedicina i Cirurgia

Protocol for the in vitro isolation and culture of mature adipocytes and white adipose tissue explants from humans and mice

  • Dades identificatives

    Identificador:  imarina:9436265
    Handle: https://hdl.handle.net/20.500.11797/imarina9436265
    Autors:  Villanueva-Carmona, Teresa; Cedo, Lidia; Nunez-Roa, Catalina; Maymo-Masip, Elsa; Vendrell, Joan; Fernandez-Veledo, Sonia
    Resum:
    White adipose tissue (WAT) explants culture allows the study of this tissue ex vivo, maintaining its structure and properties. Concurrently, isolating mature adipocytes facilitates research into fat cell metabolism and hormonal regulation. Here, we present a protocol for obtaining, isolating, and processing mature adipocytes, alongside the cultivation of WAT explants from humans and mice. We describe steps for WAT retrieval, culturing of WAT explants, WAT digestion, and adipocytes separation. We then detail procedures for culturing isolated mature adipocytes. For complete details on the use and execution of this protocol, please refer to Villanueva-Carmona et al. (2023).1

  • Altres:

    Enllaç font original: https://www.sciencedirect.com/science/article/pii/S2666166723006603
    Referència de l'ítem segons les normes APA: Villanueva-Carmona, Teresa; Cedo, Lidia; Nunez-Roa, Catalina; Maymo-Masip, Elsa; Vendrell, Joan; Fernandez-Veledo, Sonia (2023). Protocol for the in vitro isolation and culture of mature adipocytes and white adipose tissue explants from humans and mice. Star Protocols, 4(4), 102693-. DOI: 10.1016/j.xpro.2023.102693
    Referència a l'article segons font original: Star Protocols. 4 (4): 102693-
    DOI de l'article: 10.1016/j.xpro.2023.102693
    Any de publicació de la revista: 2023
    Entitat: Universitat Rovira i Virgili
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Data d'alta del registre: 2025-02-01
    Autor/s de la URV: Maymo Masip, Elsa / Vendrell Ortega, Juan José
    Departament: Bioquímica i Biotecnologia, Medicina i Cirurgia
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Tipus de publicació: Journal Publications
    Autor segons l'article: Villanueva-Carmona, Teresa; Cedo, Lidia; Nunez-Roa, Catalina; Maymo-Masip, Elsa; Vendrell, Joan; Fernandez-Veledo, Sonia
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    Àrees temàtiques: Biochemical research methods, Biochemistry, genetics and molecular biology (all), Biochemistry, genetics and molecular biology (miscellaneous), Immunology and microbiology (all), Immunology and microbiology (miscellaneous), Neuroscience (all), Neuroscience (miscellaneous)
    Adreça de correu electrònic de l'autor: jvortega@iispv.cat, elsa.maymo@urv.cat

  • Paraules clau:

    Adipocytes
    Adipose tissue
    white
    Animals
    Cell culture
    Cell isolation
    Humans
    Metabolism
    Mice
    Model organisms
    Biochemical Research Methods
    Biochemistry
    Genetics and Molecular Biology (Miscellaneous)
    Immunology and Microbiology (Miscellaneous)
    Neuroscience (Miscellaneous)
    genetics and molecular biology (all)
    Immunology and microbiology (all)
    Neuroscience (all)

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