Articles producció científicaMedicina i Cirurgia

Protocol for the in vitro isolation and culture of mature adipocytes and white adipose tissue explants from humans and mice

  • Datos identificativos

    Identificador:  imarina:9436265
    Handle: https://hdl.handle.net/20.500.11797/imarina9436265
    Autores:  Villanueva-Carmona, Teresa; Cedo, Lidia; Nunez-Roa, Catalina; Maymo-Masip, Elsa; Vendrell, Joan; Fernandez-Veledo, Sonia
    Resumen:
    White adipose tissue (WAT) explants culture allows the study of this tissue ex vivo, maintaining its structure and properties. Concurrently, isolating mature adipocytes facilitates research into fat cell metabolism and hormonal regulation. Here, we present a protocol for obtaining, isolating, and processing mature adipocytes, alongside the cultivation of WAT explants from humans and mice. We describe steps for WAT retrieval, culturing of WAT explants, WAT digestion, and adipocytes separation. We then detail procedures for culturing isolated mature adipocytes. For complete details on the use and execution of this protocol, please refer to Villanueva-Carmona et al. (2023).1

  • Otros:

    Enlace a la fuente original: https://www.sciencedirect.com/science/article/pii/S2666166723006603
    Referencia de l'ítem segons les normes APA: Villanueva-Carmona, Teresa; Cedo, Lidia; Nunez-Roa, Catalina; Maymo-Masip, Elsa; Vendrell, Joan; Fernandez-Veledo, Sonia (2023). Protocol for the in vitro isolation and culture of mature adipocytes and white adipose tissue explants from humans and mice. Star Protocols, 4(4), 102693-. DOI: 10.1016/j.xpro.2023.102693
    Referencia al articulo segun fuente origial: Star Protocols. 4 (4): 102693-
    DOI del artículo: 10.1016/j.xpro.2023.102693
    Año de publicación de la revista: 2023
    Entidad: Universitat Rovira i Virgili
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Fecha de alta del registro: 2025-02-01
    Autor/es de la URV: Maymo Masip, Elsa / Vendrell Ortega, Juan José
    Departamento: Bioquímica i Biotecnologia, Medicina i Cirurgia
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Tipo de publicación: Journal Publications
    Autor según el artículo: Villanueva-Carmona, Teresa; Cedo, Lidia; Nunez-Roa, Catalina; Maymo-Masip, Elsa; Vendrell, Joan; Fernandez-Veledo, Sonia
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Áreas temáticas: Biochemical research methods, Biochemistry, genetics and molecular biology (all), Biochemistry, genetics and molecular biology (miscellaneous), Immunology and microbiology (all), Immunology and microbiology (miscellaneous), Neuroscience (all), Neuroscience (miscellaneous)
    Direcció de correo del autor: jvortega@iispv.cat, elsa.maymo@urv.cat

  • Palabras clave:

    Adipocytes
    Adipose tissue
    white
    Animals
    Cell culture
    Cell isolation
    Humans
    Metabolism
    Mice
    Model organisms
    Biochemical Research Methods
    Biochemistry
    Genetics and Molecular Biology (Miscellaneous)
    Immunology and Microbiology (Miscellaneous)
    Neuroscience (Miscellaneous)
    genetics and molecular biology (all)
    Immunology and microbiology (all)
    Neuroscience (all)

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