Author, as appears in the article.: López-Martínez, G. Margalef-Català, M. Salinas, F. Liti, G. Cordero-Otero, R.
Department: Bioquímica i Biotecnologia
URV's Author/s: LÓPEZ MARTÍNEZ, GEMA ISABEL MARGALEF CATALÀ, MARIA DEL MAR Salinas, F. Liti, G. CORDERO OTERO, RICARDO ROMÁN
Keywords: anhydrobiosis article ATG18 gene
Abstract: Recently, different dehydration-based technologies have been evaluated for the purpose of cell and tissue preservation. Although some early results have been promising, they have not satisfied the requirements for large-scale applications. The long experience of using quantitative trait loci (QTLs) with the yeast Saccharomyces cerevisiae has proven to be a good model organism for studying the link between complex phenotypes and DNA variations. Here, we use QTL analysis as a tool for identifying the specific yeast traits involved in dehydration stress tolerance. Three hybrids obtained from stable haploids and sequenced in the Saccharomyces Genome Resequencing Project showed intermediate dehydration tolerance in most cases. The dehydration resistance trait of 96 segregants from each hybrid was quantified. A smooth, continuous distribution of the anhydrobiosis tolerance trait was found, suggesting that this trait is determined by multiple QTLs. Therefore, we carried out a QTL analysis to identify the determinants of this dehydration tolerance trait at the genomic level. Among the genes identified after reciprocal hemizygosity assays, RSM22, ATG18 and DBR1 had not been referenced in previous studies. We report new phenotypes for these genes using a previously validated test. Finally, our data illustrates the power of this approach in the investigation of the complex cell dehydration phenotype. © 2015 López-Martínez et al.
Research group: Biotecnologia Microbiana dels Aliments Biotecnologia Enològica
Thematic Areas: Bioquímica i biotecnologia Bioquímica y tecnología Biochemistry and technology
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 1932-6203
Author identifier: n/a n/a n/a 0000-0002-2318-0775 0000-0001-5402-1278
Record's date: 2015-04-27
Journal volume: 10
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0119606
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Article's DOI: 10.1371/journal.pone.0119606
Entity: Universitat Rovira i Virgili
Journal publication year: 2015
First page: 119606
Publication Type: Article Artículo Article