Author, as appears in the article.: Ricardo Closa- Monasterolo; Peter Rzehak; Richard Saffery; Eva Reischl; Marcela Covic; Simone Wahl; Veit Grote; Annick Xhonneux; Jean-Paul Langhendries; Natalia Ferre; Elvira Verduci; Enrica Riva; Piotr Socha; Dariusz Gruszfeld; Berthold Koletzko
Department: Medicina i Cirurgia
URV's Author/s: CLOSA MONASTEROLO, RICARDO; Peter Rzehak; Richard Saffery; Eva Reischl; Marcela Covic; Simone Wahl; Veit Grote; Annick Xhonneux; Jean-Paul Langhendries; Natalia Ferre; Elvira Verduci; Enrica Riva; Piotr Socha; Dariusz Gruszfeld; Berthold Koletzko
Keywords: Està en blanc
Abstract: Mounting evidence links prenatal exposure to maternal tobacco smoking with disruption of DNA methylation (DNAm) profile in the blood of infants. However, data on the postnatal stability of such DNAm signatures in childhood, as assessed by Epigenome Wide Association Studies (EWAS), are scarce. Objectives of this study were to investigate DNAm signatures associated with in utero tobacco smoke exposure beyond the 12th week of gestation in whole blood of children at age 5.5 years, to replicate previous findings in young European and American children and to assess their biological role by exploring databases and enrichment analysis. DNA methylation was measured in blood of 366 children of the multicentre European Childhood Obesity Project Study using the Illumina Infinium HM450 Beadchip (HM450K). An EWAS was conducted using linear regression of methylation values at each CpG site against in utero smoke exposure, adjusted for study characteristics, biological and technical effects. Methylation levels at five HM450K probes in MYO1G (cg12803068, cg22132788, cg19089201), CNTNAP2 (cg25949550), and FRMD4A (cg11813497) showed differential methylation that reached epigenome-wide significance according to the false-discovery-rate (FDR) criteria (q-value<0.05). Whereas cg25949550 showed decreased methylation (-2% DNAm β-value), increased methylation was observed for the other probes (9%: cg12803068; 5%: cg22132788; 4%: cg19089201 and 4%: cg11813497) in exposed relative to non-exposed subjects. This study thus replicates previous findings in children ages 3 to 5, 7 and 17 and confirms the postnatal stability of MYO1G, CNTNAP2 and FRMD4A differential methylation. The role of this differential methylation in mediating childhood phenotypes, previously associated with maternal smoking, requires further investigation.
Research group: Unitat de Recerca en Pediatria, Nutrició i Desenvolupament Humà
Thematic Areas: Ciències de la salut Ciencias de la salud Health sciences
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 1932-6203
Author identifier: N/D; N/D; N/D; N/D; N/D; N/D; N/D; N/D; N/D; N/D; N/D; N/D; N/D; N/D; N/D
Record's date: 2016-06-10
Journal volume: 11
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0155554
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Article's DOI: 10.1371/journal.pone.0155554
Entity: Universitat Rovira i Virgili
Journal publication year: 2016
First page: Article number e0155554
Publication Type: Article Artículo Article
Repositori URV