Articles producció científica> Química Física i Inorgànica

Surface-Enhanced Raman Scattering Surface Selection Rules for the Proteomic Liquid Biopsy in Real Samples: Efficient Detection of the Oncoprotein c-MYC

  • Identification data

    Identifier: PC:1978
    Handle: https://hdl.handle.net/20.500.11797/PC1978
    Authors:
    Ramon A. Alvarez-PueblaElena PazosManuel Garcia-AlgarCristina PenasMoritz NazarenusArnau TorruellaNicolas Pazos-PerezLuca GuerriniM. Eugenio VazquezEduardo Garcia-RicoJose L. Mascareñas
    Abstract:
    DOI: 10.1021/jacs.6b08957 URL: http://pubs.acs.org/doi/abs/10.1021/jacs.6b08957 Filiació URV: SI Inclòs a la memòiria: SI

  • Others:

    Author, as appears in the article.: Ramon A. Alvarez-Puebla; Elena Pazos; Manuel Garcia-Algar; Cristina Penas; Moritz Nazarenus; Arnau Torruella; Nicolas Pazos-Perez; Luca Guerrini; M. Eugenio Vazquez; Eduardo Garcia-Rico; Jose L. Mascareñas
    Department: Química Física i Inorgànica
    URV's Author/s: ALVAREZ PUEBLA, RAMON ANGEL; Elena Pazos; Manuel Garcia-Algar; Cristina Penas; Moritz Nazarenus; Arnau Torruella; Nicolas Pazos-Perez; Luca Guerrini; M. Eugenio Vazquez; Eduardo Garcia-Rico; Jose L. Mascareñas
    Keywords: Biopsy Raman scattering Surface enhanced Raman Scattering
    Abstract: Blood-based biomarkers (liquid biopsy) offer extremely valuable tools for the noninvasive diagnosis and monitoring of tumors. The protein c-MYC, a transcription factor that has been shown to be deregulated in up to 70% of human cancers, can be used as a robust proteomic signature for cancer. Herein, we developed a rapid, highly specific, and sensitive surface-enhanced Raman scattering (SERS) assay for the quantification of c-MYC in real blood samples. The sensing scheme relies on the use of specifically designed hybrid plasmonic materials and their bioderivatization with a selective peptidic receptor modified with a SERS transducer. Peptide/c-MYC recognition events translate into measurable alterations of the SERS spectra associated with a molecular reorientation of the transducer, in agreement with the surface selection rules. The efficiency of the sensor is demonstrated in cellular lines, healthy donors and a cancer patient.
    Research group: Grupo de Plasmonica y Ultradetección
    Thematic Areas: Química Química Chemistry
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 0002-7863
    Author identifier: 0000-0003-4770-5756; 0000-0001-9397-2703; n/a; n/a; n/a; n/a; n/a; 0000-0002-2925-1562; n/a; n/a; 0000-0002-7789-700X
    Record's date: 2016-11-25
    Last page: 14209
    Journal volume: 138
    Papper version: info:eu-repo/semantics/acceptedVersion
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2016
    First page: 14206
    Publication Type: Article Artículo Article

  • Keywords:

    Espectroscòpia Raman amplificada en superfície
    Biòpsia
    Sang -- Anàlisi
    Biopsy
    Raman scattering
    Surface enhanced Raman Scattering
    Química
    Química
    Chemistry
    0002-7863

  • Documents:

    DocumentPrincipal

  • Cerca a google

    Search to google scholar