Author, as appears in the article.: Ramon A. Alvarez-Puebla; Elena Pazos; Manuel Garcia-Algar; Cristina Penas; Moritz Nazarenus; Arnau Torruella; Nicolas Pazos-Perez; Luca Guerrini; M. Eugenio Vazquez; Eduardo Garcia-Rico; Jose L. Mascareñas
Department: Química Física i Inorgànica
URV's Author/s: ALVAREZ PUEBLA, RAMON ANGEL; Elena Pazos; Manuel Garcia-Algar; Cristina Penas; Moritz Nazarenus; Arnau Torruella; Nicolas Pazos-Perez; Luca Guerrini; M. Eugenio Vazquez; Eduardo Garcia-Rico; Jose L. Mascareñas
Keywords: Biopsy Raman scattering Surface enhanced Raman Scattering
Abstract: Blood-based biomarkers (liquid biopsy) offer extremely valuable tools for the noninvasive diagnosis and monitoring of tumors. The protein c-MYC, a transcription factor that has been shown to be deregulated in up to 70% of human cancers, can be used as a robust proteomic signature for cancer. Herein, we developed a rapid, highly specific, and sensitive surface-enhanced Raman scattering (SERS) assay for the quantification of c-MYC in real blood samples. The sensing scheme relies on the use of specifically designed hybrid plasmonic materials and their bioderivatization with a selective peptidic receptor modified with a SERS transducer. Peptide/c-MYC recognition events translate into measurable alterations of the SERS spectra associated with a molecular reorientation of the transducer, in agreement with the surface selection rules. The efficiency of the sensor is demonstrated in cellular lines, healthy donors and a cancer patient.
Research group: Grupo de Plasmonica y Ultradetección
Thematic Areas: Química Química Chemistry
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 0002-7863
Author identifier: 0000-0003-4770-5756; 0000-0001-9397-2703; n/a; n/a; n/a; n/a; n/a; 0000-0002-2925-1562; n/a; n/a; 0000-0002-7789-700X
Record's date: 2016-11-25
Last page: 14209
Journal volume: 138
Papper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: http://pubs.acs.org/doi/abs/10.1021/jacs.6b08957
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Article's DOI: 10.1021/jacs.6b08957
Entity: Universitat Rovira i Virgili
Journal publication year: 2016
First page: 14206
Publication Type: Article Artículo Article
Repositori URV