Autor según el artículo: Ramon A. Alvarez-Puebla; Elena Pazos; Manuel Garcia-Algar; Cristina Penas; Moritz Nazarenus; Arnau Torruella; Nicolas Pazos-Perez; Luca Guerrini; M. Eugenio Vazquez; Eduardo Garcia-Rico; Jose L. Mascareñas

Departamento: Química Física i Inorgànica

Autor/es de la URV: ALVAREZ PUEBLA, RAMON ANGEL; Elena Pazos; Manuel Garcia-Algar; Cristina Penas; Moritz Nazarenus; Arnau Torruella; Nicolas Pazos-Perez; Luca Guerrini; M. Eugenio Vazquez; Eduardo Garcia-Rico; Jose L. Mascareñas

Palabras clave: Biopsy Raman scattering Surface enhanced Raman Scattering

Resumen: Blood-based biomarkers (liquid biopsy) offer extremely valuable tools for the noninvasive diagnosis and monitoring of tumors. The protein c-MYC, a transcription factor that has been shown to be deregulated in up to 70% of human cancers, can be used as a robust proteomic signature for cancer. Herein, we developed a rapid, highly specific, and sensitive surface-enhanced Raman scattering (SERS) assay for the quantification of c-MYC in real blood samples. The sensing scheme relies on the use of specifically designed hybrid plasmonic materials and their bioderivatization with a selective peptidic receptor modified with a SERS transducer. Peptide/c-MYC recognition events translate into measurable alterations of the SERS spectra associated with a molecular reorientation of the transducer, in agreement with the surface selection rules. The efficiency of the sensor is demonstrated in cellular lines, healthy donors and a cancer patient.

Grupo de investigación: Grupo de Plasmonica y Ultradetección

Áreas temáticas: Química Química Chemistry

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 0002-7863

Identificador del autor: 0000-0003-4770-5756; 0000-0001-9397-2703; n/a; n/a; n/a; n/a; n/a; 0000-0002-2925-1562; n/a; n/a; 0000-0002-7789-700X

Fecha de alta del registro: 2016-11-25

Página final: 14209

Volumen de revista: 138

Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion

Enlace a la fuente original: http://pubs.acs.org/doi/abs/10.1021/jacs.6b08957

DOI del artículo: 10.1021/jacs.6b08957

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2016

Página inicial: 14206

Tipo de publicación: Article Artículo Article