Author, as appears in the article.: Santafé, M.M. Garcia, N. Tomàs, M. Obis, T. Lanuza M.A. Besalduch N. Tomàs J.
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Manel M. Santafé; Neus Garcia; Marta Tomàs; Teresa Obis; Maria A. Lanuza; Nuria Besalduch; Josep Tomàs
Abstract: We conducted an electrophysiological study of the functional link between the tropomyosin-related kinase B (trkB) receptor signaling mechanism and serine-threonine kinases, both protein kinase C (PKC) and protein kinase A (PKA). We describe their coordinated role in transmitter release at the neuromuscular junction (NMJ) of the Levator auris longus muscle of the adult mouse. The trkB receptor normally seems to be coupled to stimulate ACh release because inhibiting the trkB receptor with K-252a results in a significant reduction in the size of EPPs. We found that the intracellular PKC pathway can operate as in basal conditions (to potentiate ACh release) without the involvement of the trkB receptor function, although the trkB pathway needs an operative PKC pathway if it is to couple to the release mechanism and potentiate it. To actively stimulate PKA (which also results in ACh release potentiation), the operativity of trkB is a necessary condition, and one effect of trkB may be PKA stimulation.
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 0304-3940
Last page: 175
Journal volume: 251
Papper version: info:eu-repo/semantics/submittedVersion
Link to the original source: http://www.sciencedirect.com/science/article/pii/S030439401400010X
Article's DOI: 10.1016/j.neulet.2013.12.073
Entity: Universitat Rovira i Virgili.
Journal publication year: 2014
First page: 171
Repositori URV