Articles producció científica> Ciències Mèdiques Bàsiques

The interaction between tropomyosin-related kinase B receptors and serine kinases modulates acetylcholine release in adult neuromuscular junctions

  • Datos identificativos

    Identificador: PC:973
    Handle: http://hdl.handle.net/20.500.11797/PC973
  • Autores:

    Santafé, M.M.
    Garcia, N.
    Tomàs, M.
    Obis, T.
    Lanuza M.A.
    Besalduch N.
    Tomàs J.
  • Otros:

    Autor según el artículo: Santafé, M.M. Garcia, N. Tomàs, M. Obis, T. Lanuza M.A. Besalduch N. Tomàs J.
    Departamento: Ciències Mèdiques Bàsiques
    Autor/es de la URV: Manel M. Santafé; Neus Garcia; Marta Tomàs; Teresa Obis; Maria A. Lanuza; Nuria Besalduch; Josep Tomàs
    Resumen: We conducted an electrophysiological study of the functional link between the tropomyosin-related kinase B (trkB) receptor signaling mechanism and serine-threonine kinases, both protein kinase C (PKC) and protein kinase A (PKA). We describe their coordinated role in transmitter release at the neuromuscular junction (NMJ) of the Levator auris longus muscle of the adult mouse. The trkB receptor normally seems to be coupled to stimulate ACh release because inhibiting the trkB receptor with K-252a results in a significant reduction in the size of EPPs. We found that the intracellular PKC pathway can operate as in basal conditions (to potentiate ACh release) without the involvement of the trkB receptor function, although the trkB pathway needs an operative PKC pathway if it is to couple to the release mechanism and potentiate it. To actively stimulate PKA (which also results in ACh release potentiation), the operativity of trkB is a necessary condition, and one effect of trkB may be PKA stimulation.
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 0304-3940
    Página final: 175
    Volumen de revista: 251
    Versión del articulo depositado: info:eu-repo/semantics/submittedVersion
    Enlace a la fuente original: http://www.sciencedirect.com/science/article/pii/S030439401400010X
    DOI del artículo: 10.1016/j.neulet.2013.12.073
    Entidad: Universitat Rovira i Virgili.
    Año de publicación de la revista: 2014
    Página inicial: 171