Articles producció científica> Enginyeria Electrònica, Elèctrica i Automàtica

Metabolic Profiling in Formalin-Fixed and Paraffin-Embedded Prostate Cancer Tissues

  • Identification data

    Identifier: imarina:5130959
    Handle: http://hdl.handle.net/20.500.11797/imarina5130959
  • Authors:

    Cacciatore S
    Zadra G
    Bango C
    Penney K
    Tyekucheva S
    Yanes O
    Loda M
  • Others:

    Author, as appears in the article.: Cacciatore S; Zadra G; Bango C; Penney K; Tyekucheva S; Yanes O; Loda M
    Department: Enginyeria Electrònica, Elèctrica i Automàtica
    URV's Author/s: Yanes Torrado, Óscar
    Abstract: Metabolite profiling has significantly contributed to a deeper understanding of the biochemical metabolic networks and pathways in cancer cells. Metabolomics-based biomarker discovery would greatly benefit from the ability to interrogate retrospective annotated clinical specimens archived as formalin-fixed, paraffinembedded (FFPE) material. Mass spectrometry-based metabolomic analysis was performed in matched frozen and FFPE human prostate cancers as well as isogenic prostate cancer cell lines. A total of 352 and 460 metabolites were profiled in human tissues and cell lines, respectively. Classes and physical-chemical characteristics of the metabolites preserved in FFPE material were characterized and related to their preservation or loss following fixation and embedding. Metabolite classes were differentially preserved in archival FFPE tissues, regardless of the age of the block, compared with matched frozen specimen, ranging from maximal preservation of fatty acids (78%) to loss of the majority of peptides and steroids. Generally, FFPE samples showed a decrease of metabolites with functional groups, such as carboxamide. As an adjunct technique, metabolic profiles were also obtained in situ from FFPE tissue sections where metabolites were extracted in a manner that preserves tissue architecture. Despite the fact that selected metabolites were not retained after processing, global metabolic profiles obtained from FFPE can be used to predict biologic states and study biologic pathways. These results pave the way for metabolomics-based biomarker discovery/validation utilizing retrospective and clinically annotated FFPE collections.
    Thematic Areas: Oncology Odontología Molecular biology Medicine (all) Medicina iii Medicina ii Medicina i Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Cell biology Cancer research Biotecnología
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: oscar.yanes@urv.cat
    Author identifier: 0000-0003-3695-7157
    Record's date: 2023-07-31
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://mcr.aacrjournals.org/content/15/4/439
    Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Molecular Cancer Research. 15 (4): 439-447
    APA: Cacciatore S; Zadra G; Bango C; Penney K; Tyekucheva S; Yanes O; Loda M (2017). Metabolic Profiling in Formalin-Fixed and Paraffin-Embedded Prostate Cancer Tissues. Molecular Cancer Research, 15(4), 439-447. DOI: 10.1158/1541-7786.MCR-16-0262
    Article's DOI: 10.1158/1541-7786.MCR-16-0262
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2017
    Publication Type: Journal Publications
  • Keywords:

    Cancer Research,Cell Biology,Molecular Biology,Oncology
    Oncology
    Odontología
    Molecular biology
    Medicine (all)
    Medicina iii
    Medicina ii
    Medicina i
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Cell biology
    Cancer research
    Biotecnología
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