Author, as appears in the article.: Latif-Eugenin, Fadua; Beaz-Hidalgo, Roxana; Jose Figueras, Maria;
Department: Ciències Mèdiques Bàsiques
URV's Author/s: BEAZ HIDALGO, ROXANA / Figueras Salvat, María Josefa
Keywords: aeromonas detection gcat pcr Aeromonas Aerornonas Antimicrobial susceptibility Detection Fish Fresh-water Gcat Genes Genus aeromonas Gm pcr Infection Pcr Schubertii Snakehead Spp. Virulence factors Water
Abstract: The genus Aeromonas is present in a wide variety of water environments and is recognised as potentially pathogenic to humans and animals. Members of this genus are often confused with Vibrio when using automated, commercial identification systems that are culture-dependent. This study describes a polymerase chain reaction (PCR) detection method for Aeromonas that is culture-independent and that targets the glycerophospholopid-cholesterol acyltransferase (gcat) gene, which is specific for this genus. The GCAT-PCR was 100% specific in artificially inoculated water samples, with a detection limit that ranged from 2.5 to 25 cfu/mL. The success at detecting this pathogen in 86 water samples using the GCAT-PCR method was identical to the conventional culturing method when a pre-enrichment step was carried out, yielding 83.7% positive samples. On the other hand, without a pre-enrichment step. only 77.9% of the samples were positive by culturing and only 15.1% with the GCAT-CR. However, 83.7% positive samples were obtained for the GCAT-PCR when the water volume for the DNA extraction was increased from 400 mu L to 4 mL. The proposed molecular method is much faster (5 or 29 h) than the culturing method (24 or 48 h) whether performed directly or after a pre-enrichment step and it will enable the fast detection of Aeromonas in water samples helping to prevent a possible transmission to humans.
Thematic Areas: Food science Food science & technology
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: mariajose.figueras@urv.cat
ISSN: 22397132
Author identifier: 0000-0002-2268-8980
Record's date: 2023-03-05
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.pagepressjournals.org/index.php/ijfs/article/view/ijfs.2016.5489
Papper original source: Italian Journal Of Food Safety. 5 (1): 11-14
APA: Latif-Eugenin, Fadua; Beaz-Hidalgo, Roxana; Jose Figueras, Maria; (2016). A culture independent method for the detection of Aeromonas sp. from water samples. Italian Journal Of Food Safety, 5(1), 11-14. DOI: 10.4081/ijfs.2016.5489
Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
Article's DOI: 10.4081/ijfs.2016.5489
Entity: Universitat Rovira i Virgili
Journal publication year: 2016
Publication Type: Journal Publications
Repositori URV