Articles producció científica> Ciències Mèdiques Bàsiques

A culture independent method for the detection of Aeromonas sp. from water samples

  • Datos identificativos

    Identificador: imarina:5895858
    Autores:
    Latif-Eugenin, FaduaBeaz-Hidalgo, RoxanaJose Figueras, Maria
    Resumen:
    The genus Aeromonas is present in a wide variety of water environments and is recognised as potentially pathogenic to humans and animals. Members of this genus are often confused with Vibrio when using automated, commercial identification systems that are culture-dependent. This study describes a polymerase chain reaction (PCR) detection method for Aeromonas that is culture-independent and that targets the glycerophospholopid-cholesterol acyltransferase (gcat) gene, which is specific for this genus. The GCAT-PCR was 100% specific in artificially inoculated water samples, with a detection limit that ranged from 2.5 to 25 cfu/mL. The success at detecting this pathogen in 86 water samples using the GCAT-PCR method was identical to the conventional culturing method when a pre-enrichment step was carried out, yielding 83.7% positive samples. On the other hand, without a pre-enrichment step. only 77.9% of the samples were positive by culturing and only 15.1% with the GCAT-CR. However, 83.7% positive samples were obtained for the GCAT-PCR when the water volume for the DNA extraction was increased from 400 mu L to 4 mL. The proposed molecular method is much faster (5 or 29 h) than the culturing method (24 or 48 h) whether performed directly or after a pre-enrichment step and it will enable the fast detection of Aeromonas in water samples helping to prevent a possible transmission to humans.
  • Otros:

    Autor según el artículo: Latif-Eugenin, Fadua; Beaz-Hidalgo, Roxana; Jose Figueras, Maria;
    Departamento: Ciències Mèdiques Bàsiques
    Autor/es de la URV: BEAZ HIDALGO, ROXANA / Figueras Salvat, María Josefa
    Palabras clave: aeromonas detection gcat pcr Aeromonas Aerornonas Antimicrobial susceptibility Detection Fish Fresh-water Gcat Genes Genus aeromonas Gm pcr Infection Pcr Schubertii Snakehead Spp. Virulence factors Water
    Resumen: The genus Aeromonas is present in a wide variety of water environments and is recognised as potentially pathogenic to humans and animals. Members of this genus are often confused with Vibrio when using automated, commercial identification systems that are culture-dependent. This study describes a polymerase chain reaction (PCR) detection method for Aeromonas that is culture-independent and that targets the glycerophospholopid-cholesterol acyltransferase (gcat) gene, which is specific for this genus. The GCAT-PCR was 100% specific in artificially inoculated water samples, with a detection limit that ranged from 2.5 to 25 cfu/mL. The success at detecting this pathogen in 86 water samples using the GCAT-PCR method was identical to the conventional culturing method when a pre-enrichment step was carried out, yielding 83.7% positive samples. On the other hand, without a pre-enrichment step. only 77.9% of the samples were positive by culturing and only 15.1% with the GCAT-CR. However, 83.7% positive samples were obtained for the GCAT-PCR when the water volume for the DNA extraction was increased from 400 mu L to 4 mL. The proposed molecular method is much faster (5 or 29 h) than the culturing method (24 or 48 h) whether performed directly or after a pre-enrichment step and it will enable the fast detection of Aeromonas in water samples helping to prevent a possible transmission to humans.
    Áreas temáticas: Food science Food science & technology
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: mariajose.figueras@urv.cat
    ISSN: 22397132
    Identificador del autor: 0000-0002-2268-8980
    Fecha de alta del registro: 2023-03-05
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://www.pagepressjournals.org/index.php/ijfs/article/view/ijfs.2016.5489
    Referencia al articulo segun fuente origial: Italian Journal Of Food Safety. 5 (1): 11-14
    Referencia de l'ítem segons les normes APA: Latif-Eugenin, Fadua; Beaz-Hidalgo, Roxana; Jose Figueras, Maria; (2016). A culture independent method for the detection of Aeromonas sp. from water samples. Italian Journal Of Food Safety, 5(1), 11-14. DOI: 10.4081/ijfs.2016.5489
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI del artículo: 10.4081/ijfs.2016.5489
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2016
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Food Science,Food Science & Technology
    aeromonas
    detection
    gcat
    pcr
    Aeromonas
    Aerornonas
    Antimicrobial susceptibility
    Detection
    Fish
    Fresh-water
    Gcat
    Genes
    Genus aeromonas
    Gm pcr
    Infection
    Pcr
    Schubertii
    Snakehead
    Spp.
    Virulence factors
    Water
    Food science
    Food science & technology
    22397132
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