Author, as appears in the article.: Martínez-Micaelo N; Beltrán-Debón R; Baiges I; Faiges M; Alegret J

Department: Ciències Mèdiques Bàsiques Bioquímica i Biotecnologia

URV's Author/s: Alegret Colomé, Josep Maria / BAIGES BLANCO, ISABEL MARIA / Beltrán Debón, Raúl Alejandro / Martínez Micaelo, Nieves Beatriz

Keywords: Smooth-muscle-cells Mir-718 Micrornas Microrna Identification Discovery Dilation Differential expression Calcification Bicuspid aortic valve Beta Bav bicuspid aortic valve Aortopathy Aortic dilation Angiogenesis

Abstract: © 2017 The Author(s). Background: We aimed to determine the circulating miRNA expression profile associated with BAV and aortic dilation to provide diagnostic and prognostic biomarkers for BAV and/or aortic dilation. Methods and results: We applied a miRNome-wide microarray approach using plasma samples (n = 24) from healthy tricuspid aortic valve individuals, BAV patients and BAV patients with aortic dilation to compare and identify the specific miRNAs associated with BAV and aortic dilation. In a second stage, the expression patterns of the miRNA candidates were validated by RT-qPCR in an independent cohort (n = 43). The miRNA microarray data and RT-qPCR analyses revealed that the expression levels of circulating miR-122, miR-130a and miR-486 are significantly influenced by the morphology of the aortic valve (bicuspid/tricuspid) and could be functionally involved in the regulation of TGF-β1 signalling. Furthermore, the expression pattern of miR-718 in the plasma was strongly influenced by dilation of the ascending aorta. miR-718 expression was inversely correlated with the aortic diameter (R = -0.63, p = 3.1 × 10-5) and was an independent predictor of aortic dilation (β = -0.41, p = 0.022). The genes targeted by miR-718 are involved in the regulation of vascular remodelling. Conclusions: We propose that miR-122, miR-130a, miR-486 and miR-718 are new molecular features associated with BAV and aortic dilation principally by the activation of TGF-β1 pathway and vascular remodelling mediated by VEGF signalling pathways.

Thematic Areas: Odontología Nutrição Medicine, research & experimental Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar General medicine General biochemistry,genetics and molecular biology Farmacia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Biotecnología Biochemistry, genetics and molecular biology (miscellaneous) Biochemistry, genetics and molecular biology (all)

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 14795876

Author's mail: neus.martinez@urv.cat raul.beltran@urv.cat josepmaria.alegret@urv.cat

Author identifier: 0000-0001-9691-1906 0000-0002-6117-5512

Record's date: 2023-02-19

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-017-1176-x

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Journal Of Translational Medicine. 15 (1): 76-

APA: Martínez-Micaelo N; Beltrán-Debón R; Baiges I; Faiges M; Alegret J (2017). Specific circulating microRNA signature of bicuspid aortic valve disease. Journal Of Translational Medicine, 15(1), 76-. DOI: 10.1186/s12967-017-1176-x

Article's DOI: 10.1186/s12967-017-1176-x

Entity: Universitat Rovira i Virgili

Journal publication year: 2017

Publication Type: Journal Publications