Author, as appears in the article.: Pedret A; Catalán Ú; Rubió L; Baiges I; Herrero P; Piñol C; Rodríguez-Calvo R; Canela N; Fernández-Castillejo S; Motilva MJ; Solà R
Department: Bioquímica i Biotecnologia Medicina i Cirurgia
URV's Author/s: BAIGES BLANCO, ISABEL MARIA / Catalán Santos, Úrsula / FERNÁNDEZ CASTILLEJO, SARA / HERRERO GIL, POL / Pedret Figuerola, Anna / Rodríguez Calvo, Ricardo / Solà Alberich, Rosa Maria
Keywords: Tissues Secoiridoids Proteome Protein-protein interactions Phosphoproteome
Abstract: © 2020 American Chemical Society. All rights reserved. Protein functional interactions could explain the biological response of secoiridoids (SECs), main phenolic compounds in virgin olive oil (VOO). The aim was to assess protein-protein interactions (PPIs) of the aorta gap junction alpha-1 (GJA1) and the heart peptidyl-prolyl cis-trans isomerase (FKBP1A), plus the phosphorylated heart proteome, to describe new molecular pathways in the cardiovascular system in rats using nanoliquid chromatography coupled with mass spectrometry. PPIs modified by SECs and associated with GJA1 in aorta rat tissue were calpain, TUBA1A, and HSPB1. Those associated with FKBP1A in rat heart tissue included SUCLG1, HSPE1, and TNNI3. In the heart, SECs modulated the phosphoproteome through the main canonical pathways PI3K/mTOR signaling (AKT1S1 and GAB2) and gap junction signaling (GAB2 and GJA1). PPIs associated with GJA1 and with FKBP1A, the phosphorylation of GAB2, and the dephosphorylation of GJA1 and AKT1S1 in rat tissues are promising protein targets promoting cardiovascular protection to explain the health benefits of VOO.
Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Planejamento urbano e regional / demografia Odontología Nutrição Medicina veterinaria Medicina ii Medicina i Materiais Interdisciplinar General medicine General chemistry General agricultural and biological sciences Food science & technology Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Educação Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, applied Chemistry (miscellaneous) Chemistry (all) Biotecnología Biodiversidade Astronomia / física Agriculture, multidisciplinary Agriculture Agricultural and biological sciences (miscellaneous) Agricultural and biological sciences (all)
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: ursula.catalan@urv.cat ricardo.rodriguez@urv.cat anna.pedret@urv.cat rosa.sola@urv.cat
Author identifier: 0000-0001-8884-9823 0000-0002-5327-932X 0000-0002-8359-235X
Record's date: 2024-07-27
Papper version: info:eu-repo/semantics/submittedVersion
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Journal Of Agricultural And Food Chemistry. 69 (5): 1536-1554
APA: Pedret A; Catalán Ú; Rubió L; Baiges I; Herrero P; Piñol C; Rodríguez-Calvo R; Canela N; Fernández-Castillejo S; Motilva MJ; Solà R (2021). Phosphoproteomic Analysis and Protein-Protein Interaction of Rat Aorta GJA1 and Rat Heart FKBP1A after Secoiridoid Consumption from Virgin Olive Oil: A Functional Proteomic Approach. Journal Of Agricultural And Food Chemistry, 69(5), 1536-1554. DOI: 10.1021/acs.jafc.0c07164
Entity: Universitat Rovira i Virgili
Journal publication year: 2021
Publication Type: Journal Publications
Repositori URV