Autor según el artículo: Pedret, Anna; Catalan, Ursula; Rubio, Laura; Baiges, Isabel; Herrero, Pol; Pinol, Carme; Rodriguez-Calvo, Ricardo; Canela, Nuria; Fernandez-Castillejo, Sara; Motilva, Maria-Jose; Sola, Rosa
Departamento: Bioquímica i Biotecnologia; Medicina i Cirurgia
Autor/es de la URV: BAIGES BLANCO, ISABEL MARIA / Catalán Santos, Úrsula / FERNÁNDEZ CASTILLEJO, SARA / HERRERO GIL, POL / Pedret Figuerola, Anna / Rodríguez Calvo, Ricardo / Solà Alberich, Rosa Maria
Palabras clave: Tor serine-threonine kinases; Tissues; Secoiridoids; Rats, wistar; Rats; Proteomics; Proteome; Protein−protein interactions; Protein-protein interactions; Protein binding; Phosphoproteome; Phosphoproteins; Olive oil; Myocardium; Mtor protein, rat; Male; Iridoids; Gja1 protein, rat; Gab2 protein, rat; Connexin 43; Aorta; Animals; Adaptor proteins, signal transducing
Resumen: © 2020 American Chemical Society. All rights reserved. Protein functional interactions could explain the biological response of secoiridoids (SECs), main phenolic compounds in virgin olive oil (VOO). The aim was to assess protein-protein interactions (PPIs) of the aorta gap junction alpha-1 (GJA1) and the heart peptidyl-prolyl cis-trans isomerase (FKBP1A), plus the phosphorylated heart proteome, to describe new molecular pathways in the cardiovascular system in rats using nanoliquid chromatography coupled with mass spectrometry. PPIs modified by SECs and associated with GJA1 in aorta rat tissue were calpain, TUBA1A, and HSPB1. Those associated with FKBP1A in rat heart tissue included SUCLG1, HSPE1, and TNNI3. In the heart, SECs modulated the phosphoproteome through the main canonical pathways PI3K/mTOR signaling (AKT1S1 and GAB2) and gap junction signaling (GAB2 and GJA1). PPIs associated with GJA1 and with FKBP1A, the phosphorylation of GAB2, and the dephosphorylation of GJA1 and AKT1S1 in rat tissues are promising protein targets promoting cardiovascular protection to explain the health benefits of VOO.
Áreas temáticas: Zootecnia / recursos pesqueiros; Saúde coletiva; Química; Planejamento urbano e regional / demografia; Odontología; Nutrição; Medicina veterinaria; Medicina ii; Medicina i; Materiais; Interdisciplinar; General medicine; General chemistry; General agricultural and biological sciences; Food science & technology; Farmacia; Ensino; Engenharias iv; Engenharias iii; Engenharias ii; Educação; Ciências biológicas iii; Ciências biológicas ii; Ciências biológicas i; Ciências ambientais; Ciências agrárias i; Ciência de alimentos; Ciência da computação; Chemistry, applied; Chemistry (miscellaneous); Chemistry (all); Biotecnología; Biodiversidade; Astronomia / física; Agriculture, multidisciplinary; Agriculture; Agricultural and biological sciences (miscellaneous); Agricultural and biological sciences (all)
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: ricardo.rodriguez@urv.cat; ursula.catalan@urv.cat; anna.pedret@urv.cat; rosa.sola@urv.cat
Fecha de alta del registro: 2025-02-24
Versión del articulo depositado: info:eu-repo/semantics/submittedVersion
Enlace a la fuente original: https://pubs.acs.org/doi/pdf/10.1021/acs.jafc.0c07164
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Journal Of Agricultural And Food Chemistry. 69 (5): 1536-1554
Referencia de l'ítem segons les normes APA: Pedret, Anna; Catalan, Ursula; Rubio, Laura; Baiges, Isabel; Herrero, Pol; Pinol, Carme; Rodriguez-Calvo, Ricardo; Canela, Nuria; Fernandez-Castillejo (2021). Phosphoproteomic Analysis and Protein-Protein Interaction of Rat Aorta GJA1 and Rat Heart FKBP1A after Secoiridoid Consumption from Virgin Olive Oil: A Functional Proteomic Approach. Journal Of Agricultural And Food Chemistry, 69(5), 1536-1554. DOI: 10.1021/acs.jafc.0c07164
DOI del artículo: 10.1021/acs.jafc.0c07164
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2021
Tipo de publicación: Journal Publications
Repositori URV