Author, as appears in the article.: Cobo, Isidro; Matheu, M Isabel; Castillon, Sergio; Davis, Benjamin G; Boutureira, Omar
Department: Química Analítica i Química Orgànica
URV's Author/s: Boutureira Martín, Omar / Castillón Miranda, Sergio / Matheu Malpartida, María Isabel
Keywords: Sulfhydryl compounds Recombinant proteins Protein modifications Glycoproteins Glycoprotein-synthesis Dendrimers Cycloaddition reaction Bioorthogonal reactions Bioconjugations Azides Amino acids strategy protein modifications protein ligands lectin glycosylation glycoproteins glycomimetics dendrimers dehydroalanine cysteine bioorthogonal reactions adhesion
Abstract: Methods that site-selectively attach multivalent carbohydrate moieties to proteins can be used to generate homogeneous glycodendriproteins as synthetic functional mimics of glycoproteins. Here, we study aspects of the scope and limitations of some common bioconjugation techniques that can give access to well-defined glycodendriproteins. A diverse reactive platform was designed via use of thiol-Michael-type additions, thiol-ene reactions, and Cu(I)-mediated azide-alkyne cycloadditions from recombinant proteins containing the non-canonical amino acids dehydroalanine, homoallylglycine, homopropargylglycine, and azidohomoalanine.© 2022 Wiley-VCH GmbH.
Thematic Areas: Química Organic chemistry Molecular medicine Molecular biology Medicina ii Materiais Interdisciplinar General medicine Farmacia Ciências biológicas ii Ciências biológicas i Ciências ambientais Chemistry, medicinal Biotecnología Biochemistry & molecular biology Biochemistry Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: omar.boutureira@urv.cat maribel.matheu@urv.cat sergio.castillon@urv.cat
Author identifier: 0000-0002-0768-8309 0000-0001-5216-9260 0000-0002-0690-7549
Record's date: 2024-11-30
Papper version: info:eu-repo/semantics/publishedVersion
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Chembiochem. 23 (10): e202200020-
APA: Cobo, Isidro; Matheu, M Isabel; Castillon, Sergio; Davis, Benjamin G; Boutureira, Omar (2022). Probing Site-Selective Conjugation Chemistries for the Construction of Homogeneous Synthetic Glycodendriproteins. Chembiochem, 23(10), e202200020-. DOI: 10.1002/cbic.202200020
Entity: Universitat Rovira i Virgili
Journal publication year: 2022
Publication Type: Journal Publications
Repositori URV