Author, as appears in the article.: Shkembi X; Botero ML; Skouridou V; Jauset-Rubio M; Svobodova M; Ballester P; Bashammakh AS; El-Shahawi MS; Alyoubi AO; O'Sullivan CK
Department: Enginyeria Química
URV's Author/s: BOTERO GALLEGO, MARY LUZ / Jauset Rubio, Miriam / O'SULLIVAN, CIARA KATHLEEN / Shkembi, Xhensila / Skouridou, Vasoula / SVOBODOVÁ, MARKÉTA
Keywords: Selex Nandrolone Gold nanoparticle assay Doping Aptamers Androgenic steroids testosterone selex selection nortestosterone nandrolone metabolites human urine gold nanoparticle assay elisa doping assay antibodies 19-nortestosterone
Abstract: The illicit use of anabolic androgenic steroids (AAS) as performance-enhancing drugs remains a global issue threatening not only the credibility of competitive sports but also public health due to the well-documented adverse effects they elicit. AAS abuse is not restricted only to professional sports, but also extends to recreational athletes and adolescents as well as in livestock production as growth-promoting agents. Testosterone and nandrolone are among the AAS most frequently exploited. Gas chromatography-mass spectrometry is the reference method for AAS detection, but it is strictly laboratory-based and cannot be performed on-site. The great potential of aptamers in bioanalytical applications and specifically for the development of simple analytical tools suitable for on-site analysis has been extensively documented. In this report, we describe the selection and identification of aptamers binding nandrolone, exhibiting affinity dissociation constants in the low nanomolar range. A label-free colorimetric assay based on gold nanoparticles was developed using one of these novel aptamers for the detection of nandrolone and/or its metabolites. The assay could be deployed for the rapid, on-site, facile and cost-effective screening of samples and provide qualitative visual results with a red to purple/blue color change being indicative of a positive result.Copyright © 2022. Published by Elsevier Inc.
Thematic Areas: Química Odontología Molecular biology Medicina ii Medicina i Materiais Interdisciplinar Geociências General medicine Farmacia Engenharias iv Engenharias iii Engenharias ii Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Chemistry, analytical Cell biology Biotecnología Biophysics Biochemistry & molecular biology Biochemistry Biochemical research methods Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: vasoula.skouridou@urv.cat miriam.jauset@urv.cat xhensila.shkembi@estudiants.urv.cat
Author identifier: 0000-0002-9712-5429 0000-0002-9943-6132
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.sciencedirect.com/science/article/pii/S0003269722003979
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Analytical Biochemistry. 658 114937-114937
APA: Shkembi X; Botero ML; Skouridou V; Jauset-Rubio M; Svobodova M; Ballester P; Bashammakh AS; El-Shahawi MS; Alyoubi AO; O'Sullivan CK (2022). Novel nandrolone aptamer for rapid colorimetric detection of anabolic steroids. Analytical Biochemistry, 658(), 114937-114937. DOI: 10.1016/j.ab.2022.114937
Article's DOI: 10.1016/j.ab.2022.114937
Entity: Universitat Rovira i Virgili
Journal publication year: 2022
Publication Type: Journal Publications
Repositori URV