Autor según el artículo: Shkembi X; Botero ML; Skouridou V; Jauset-Rubio M; Svobodova M; Ballester P; Bashammakh AS; El-Shahawi MS; Alyoubi AO; O'Sullivan CK
Departamento: Enginyeria Química
Autor/es de la URV: BOTERO GALLEGO, MARY LUZ / Jauset Rubio, Miriam / O'SULLIVAN, CIARA KATHLEEN / Shkembi, Xhensila / Skouridou, Vasoula / SVOBODOVÁ, MARKÉTA
Palabras clave: Selex Nandrolone Gold nanoparticle assay Doping Aptamers Androgenic steroids testosterone selex selection nortestosterone nandrolone metabolites human urine gold nanoparticle assay elisa doping assay antibodies 19-nortestosterone
Resumen: The illicit use of anabolic androgenic steroids (AAS) as performance-enhancing drugs remains a global issue threatening not only the credibility of competitive sports but also public health due to the well-documented adverse effects they elicit. AAS abuse is not restricted only to professional sports, but also extends to recreational athletes and adolescents as well as in livestock production as growth-promoting agents. Testosterone and nandrolone are among the AAS most frequently exploited. Gas chromatography-mass spectrometry is the reference method for AAS detection, but it is strictly laboratory-based and cannot be performed on-site. The great potential of aptamers in bioanalytical applications and specifically for the development of simple analytical tools suitable for on-site analysis has been extensively documented. In this report, we describe the selection and identification of aptamers binding nandrolone, exhibiting affinity dissociation constants in the low nanomolar range. A label-free colorimetric assay based on gold nanoparticles was developed using one of these novel aptamers for the detection of nandrolone and/or its metabolites. The assay could be deployed for the rapid, on-site, facile and cost-effective screening of samples and provide qualitative visual results with a red to purple/blue color change being indicative of a positive result.Copyright © 2022. Published by Elsevier Inc.
Áreas temáticas: Química Odontología Molecular biology Medicina ii Medicina i Materiais Interdisciplinar Geociências General medicine Farmacia Engenharias iv Engenharias iii Engenharias ii Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Chemistry, analytical Cell biology Biotecnología Biophysics Biochemistry & molecular biology Biochemistry Biochemical research methods Astronomia / física
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: vasoula.skouridou@urv.cat miriam.jauset@urv.cat xhensila.shkembi@estudiants.urv.cat
Identificador del autor: 0000-0002-9712-5429 0000-0002-9943-6132
Fecha de alta del registro: 2024-07-20
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://www.sciencedirect.com/science/article/pii/S0003269722003979
URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Analytical Biochemistry. 658 114937-114937
Referencia de l'ítem segons les normes APA: Shkembi X; Botero ML; Skouridou V; Jauset-Rubio M; Svobodova M; Ballester P; Bashammakh AS; El-Shahawi MS; Alyoubi AO; O'Sullivan CK (2022). Novel nandrolone aptamer for rapid colorimetric detection of anabolic steroids. Analytical Biochemistry, 658(), 114937-114937. DOI: 10.1016/j.ab.2022.114937
DOI del artículo: 10.1016/j.ab.2022.114937
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2022
Tipo de publicación: Journal Publications
Repositori URV