Articles producció científica> Enginyeria Química

Novel nandrolone aptamer for rapid colorimetric detection of anabolic steroids

  • Datos identificativos

    Identificador: imarina:9282477
    Autores:
    Shkembi, XhensilaBotero, Mary LuzSkouridou, VassoJauset-Rubio, MiriamSvobodova, MarketaBallester, PabloBashammakh, Abdulaziz SEl-Shahawi, Mohammad SAlyoubi, Abdulrahman OSullivan, Ciara K O
    Resumen:
    The illicit use of anabolic androgenic steroids (AAS) as performance-enhancing drugs remains a global issue threatening not only the credibility of competitive sports but also public health due to the well-documented adverse effects they elicit. AAS abuse is not restricted only to professional sports, but also extends to recreational athletes and adolescents as well as in livestock production as growth-promoting agents. Testosterone and nandrolone are among the AAS most frequently exploited. Gas chromatography-mass spectrometry is the reference method for AAS detection, but it is strictly laboratory-based and cannot be performed on-site. The great potential of aptamers in bioanalytical applications and specifically for the development of simple analytical tools suitable for on-site analysis has been extensively documented. In this report, we describe the selection and identification of aptamers binding nandrolone, exhibiting affinity dissociation constants in the low nanomolar range. A label-free colorimetric assay based on gold nanoparticles was developed using one of these novel aptamers for the detection of nandrolone and/or its metabolites. The assay could be deployed for the rapid, on-site, facile and cost-effective screening of samples and provide qualitative visual results with a red to purple/blue color change being indicative of a positive result.Copyright © 2022. Published by Elsevier Inc.
  • Otros:

    Autor según el artículo: Shkembi, Xhensila; Botero, Mary Luz; Skouridou, Vasso; Jauset-Rubio, Miriam; Svobodova, Marketa; Ballester, Pablo; Bashammakh, Abdulaziz S; El-Shahawi, Mohammad S; Alyoubi, Abdulrahman O; Sullivan, Ciara K O
    Departamento: Enginyeria Química
    Autor/es de la URV: BOTERO GALLEGO, MARY LUZ / Jauset Rubio, Miriam / O'SULLIVAN, CIARA KATHLEEN / Shkembi, Xhensila / Skouridou, Vasoula / SVOBODOVÁ, MARKÉTA
    Palabras clave: Testosterone congeners Testosterone Selex Performance-enhancing substances Nandrolone Metal nanoparticles Humans Gold nanoparticle assay Gold Doping in sports Doping Colorimetry Aptamers Androgenic steroids Anabolic agents Adolescent testosterone selex selection nortestosterone nandrolone metabolites human urine gold nanoparticle assay elisa doping assay antibodies 19-nortestosterone
    Resumen: The illicit use of anabolic androgenic steroids (AAS) as performance-enhancing drugs remains a global issue threatening not only the credibility of competitive sports but also public health due to the well-documented adverse effects they elicit. AAS abuse is not restricted only to professional sports, but also extends to recreational athletes and adolescents as well as in livestock production as growth-promoting agents. Testosterone and nandrolone are among the AAS most frequently exploited. Gas chromatography-mass spectrometry is the reference method for AAS detection, but it is strictly laboratory-based and cannot be performed on-site. The great potential of aptamers in bioanalytical applications and specifically for the development of simple analytical tools suitable for on-site analysis has been extensively documented. In this report, we describe the selection and identification of aptamers binding nandrolone, exhibiting affinity dissociation constants in the low nanomolar range. A label-free colorimetric assay based on gold nanoparticles was developed using one of these novel aptamers for the detection of nandrolone and/or its metabolites. The assay could be deployed for the rapid, on-site, facile and cost-effective screening of samples and provide qualitative visual results with a red to purple/blue color change being indicative of a positive result.Copyright © 2022. Published by Elsevier Inc.
    Áreas temáticas: Química Odontología Molecular biology Medicina ii Medicina i Materiais Interdisciplinar Geociências General medicine Farmacia Engenharias iv Engenharias iii Engenharias ii Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Chemistry, analytical Cell biology Biotecnología Biophysics Biochemistry & molecular biology Biochemistry Biochemical research methods Astronomia / física
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: vasoula.skouridou@urv.cat miriam.jauset@urv.cat xhensila.shkembi@estudiants.urv.cat
    Identificador del autor: 0000-0002-9712-5429 0000-0002-9943-6132
    Fecha de alta del registro: 2024-10-12
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://www.sciencedirect.com/science/article/pii/S0003269722003979
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Analytical Biochemistry. 658 114937-114937
    Referencia de l'ítem segons les normes APA: Shkembi, Xhensila; Botero, Mary Luz; Skouridou, Vasso; Jauset-Rubio, Miriam; Svobodova, Marketa; Ballester, Pablo; Bashammakh, Abdulaziz S; El-Shahawi (2022). Novel nandrolone aptamer for rapid colorimetric detection of anabolic steroids. Analytical Biochemistry, 658(), 114937-114937. DOI: 10.1016/j.ab.2022.114937
    DOI del artículo: 10.1016/j.ab.2022.114937
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2022
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Biochemical Research Methods,Biochemistry,Biochemistry & Molecular Biology,Biophysics,Cell Biology,Chemistry, Analytical,Molecular Biology
    Testosterone congeners
    Testosterone
    Selex
    Performance-enhancing substances
    Nandrolone
    Metal nanoparticles
    Humans
    Gold nanoparticle assay
    Gold
    Doping in sports
    Doping
    Colorimetry
    Aptamers
    Androgenic steroids
    Anabolic agents
    Adolescent
    testosterone
    selex
    selection
    nortestosterone
    nandrolone
    metabolites
    human urine
    gold nanoparticle assay
    elisa
    doping
    assay
    antibodies
    19-nortestosterone
    Química
    Odontología
    Molecular biology
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Geociências
    General medicine
    Farmacia
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Educação física
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Chemistry, analytical
    Cell biology
    Biotecnología
    Biophysics
    Biochemistry & molecular biology
    Biochemistry
    Biochemical research methods
    Astronomia / física
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