A cocktail of histidine, carnosine, cysteine and serine reduces adiposity and improves metabolic health and adipose tissue immunometabolic function in ovariectomized rats

Author, as appears in the article.: Baudin, Julio; Hernandez-Baixauli, Julia; Romero-Gimenez, Jordi; Yang, Hong; Mulero, Francisca; Puiggros, Francesc; Mardinoglu, Adil; Arola, Lluis; Caimari, Antoni

Department: Bioquímica i Biotecnologia

URV's Author/s: Arola Fernández, Lluís / Arola Ferrer, Luis Maria / Baudin Luque, Julio Carlos

Keywords: Supplementatio; Serine; Oxidative stress; Obesity; Nutraceuticals; Nf-kappa-b; Menopause; Insulin-resistance; Inflammation; Immunometabolic health; Histidine; Food-intake; Expression; Elevated plus-maze; Cysteine; Complementary and alternative therapies; Complementary and alternative therapie; Cell function; Carnosine; Body-composition

Abstract: Many women have sought alternative therapies to address menopause. Recently, a multi-ingredient supplement (MIS) containing L-histidine, L-carnosine, L-serine, and L-cysteine has been shown to be effective at ameliorating hepatic steatosis (HS) in ovariectomized (OVX) rats, a postmenopausal oestrogen deficiency model. Considering that HS frequently accompanies obesity, which often occurs during menopause, we aimed to investigate the effects of this MIS for 8 weeks in OVX rats. Twenty OVX rats were orally supplemented with either MIS (OVX-MIS) or vehicle (OVX). Ten OVX rats received vehicle orally along with subcutaneous injections of 17 beta-oestradiol (OVX-E2), whereas 10 rats underwent a sham operation and received oral and injected vehicles (control group). MIS consumption partly counteracted the fat mass accretion observed in OVX animals, leading to decreased total fat mass, adiposity index and retroperitoneal white adipose tissue (RWAT) adipocyte hypertrophy. OVX-MIS rats also displayed increased lean mass and lean/fat ratio, suggesting a healthier body composition, similar to the results reported for OVX-E2 animals. MIS consumption decreased the circulating levels of the proinflammatory marker CRP, the total cholesterol-to-HDL-cholesterol ratio and the leptin-to-adiponectin ratio, a biomarker of diabetes risk and metabolic syndrome. RWAT transcriptomics indicated that MIS favourably regulated genes involved in adipocyte structure and morphology, cell fate determination and differentiation, glucose/insulin homeostasis, inflammation, response to stress and oxidative phosphorylation, which may be mechanisms underlying the beneficial effects described for OVX-MIS rats. Our results pave the way for using this MIS formulation to improve the body composition and immunometabolic health of menopausal women.

Thematic Areas: Saúde coletiva; Química; Pharmacology & pharmacy; Pharmacology; Odontología; Medicine, research & experimental; Medicine (miscellaneous); Medicina veterinaria; Medicina iii; Medicina ii; Medicina i; Materiais; Interdisciplinar; Farmacia; Engenharias iii; Engenharias ii; Enfermagem; Educação física; Ciências biológicas iii; Ciências biológicas ii; Ciências biológicas i; Ciências agrárias i; Ciência de alimentos; Ciência da computação; Biotecnología; Biodiversidade; Astronomia / física

licence for use: https://creativecommons.org/licenses/by/3.0/es/

Author's mail: juliocarlos.baudin@estudiants.urv.cat; lluis.arola@estudiants.urv.cat; lluis.arola@estudiants.urv.cat; lluis.arola@urv.cat

Record's date: 2025-01-28

Paper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.sciencedirect.com/science/article/pii/S0753332224012113

Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/

Paper original source: Biomedicine & Pharmacotherapy. 179 117326-

APA: Baudin, Julio; Hernandez-Baixauli, Julia; Romero-Gimenez, Jordi; Yang, Hong; Mulero, Francisca; Puiggros, Francesc; Mardinoglu, Adil; Arola, Lluis; Ca (2024). A cocktail of histidine, carnosine, cysteine and serine reduces adiposity and improves metabolic health and adipose tissue immunometabolic function in ovariectomized rats. Biomedicine & Pharmacotherapy, 179(), 117326-. DOI: 10.1016/j.biopha.2024.117326

Article's DOI: 10.1016/j.biopha.2024.117326

Entity: Universitat Rovira i Virgili

Journal publication year: 2024

Publication Type: Journal Publications