Autor según el artículo: Nadal, L.; Garcia, N.; Hurtado, E.; Simó, A.; Tomàs, M.; Lanuza, M.A.; Cilleros, V.; Tomàs, J.

Departamento: Ciències Mèdiques Bàsiques

Autor/es de la URV: NADAL MAGRIÑÀ, LAURA; Garcia, N.; HURTADO CABALLERO, ERICA; SIMÓ OLLÉ, ANNA; TOMAS MARGINET, MARTA; LANUZA ESCOLANO, MARÍA ANGEL; CILLEROS MAÑÉ, VÍCTOR; Tomàs, J.

Palabras clave: cholinergic synapses motor nerve terminal Neuromuscular junction

Resumen: The development of the nervous system involves the overproduction of synapses but connectivity is refined by Hebbian activity-dependent axonal competition. The newborn skeletal muscle fibers are polyinnervated but, at the end of the competition process, some days later, become innervated by a single axon. We used quantitative confocal imaging of the autofluorescent axons from transgenic B6.Cg-Tg (Thy1-YFP)16 Jrs/J mice to investigate the possible cooperation of the muscarinic autoreceptors (mAChR, M1-, M2- and M4-subtypes) and the tyrosine kinase B (TrkB) receptor in the control of axonal elimination after the mice Levator auris longus (LAL) muscle had been exposed to several selective antagonist of the corresponding receptor pathways in vivo. Our previous results show that M1, M2 and TrkB signaling individually increase axonal loss rate around P9. Here we show that although the M1 and TrkB receptors cooperate and add their respective individual effects to increase axonal elimination rate even more, the effect of the M2 receptor is largely independent of both M1 and TrkB receptors. Thus both, cooperative and non-cooperative signaling mechanisms contribute to developmental synapse elimination.

Grupo de investigación: Unitat d'Histologia i Neurobiologia

Áreas temáticas: Ciències de la salut Ciencias de la salud Health sciences

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 1663-4365

Identificador del autor: N/D; N/D; N/D; N/D; N/D; N/D; N/D; N/D

Fecha de alta del registro: 2017-03-21

Volumen de revista: 9

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: https://www.frontiersin.org/articles/10.3389/fnagi.2017.00024/full

DOI del artículo: 10.3389/fnagi.2017.00024

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2017

Página inicial: Art.num. 24

Tipo de publicación: Article Artículo Article