Autor según el artículo: Ginés I, Gil-Cardoso K, Robles P, Arola L, Terra X, Blay M, Ardévol A, Pinent M.

Departamento: Bioquímica i Biotecnologia

Autor/es de la URV: Ardèvol Grau, Anna / Arola Ferrer, Luis Maria / GINÉS MIR, IRIS / Pinent Armengol, Montserrat / Terra Barbadora, Ximena

Palabras clave: Transepithelial activity Gut hormone secretion Food bioactives Ex vivo model Enteroendocrine system gut hormone secretion food bioactives ex vivo model enteroendocrine system

Resumen: The enteroendocrine system coordinates gastrointestinal (GI) tract functionality and the whole organism. However, the scarcity of enteroendocrine cells and their scattered distribution make them difficult to study. Here, we glued segments of the GI wall of pigs to a silicon tube, keeping the apical and the basolateral sides separate. The fact that there was less than 1% of 70-kDa fluorescein isothiocyanate (FITC)-dextran on the basolateral side proved that the gluing was efficient. Since the lactate dehydrogenase leakage at basolateral side was lower than 0.1% (1.40 ± 0.17 nKatals) it proved that the tissue was viable. The intestinal barrier function was maintained as it is in segments mounted in Ussing chambers (the amount of Lucifer Yellow crossing it, was similar between them; respectively, % LY, 0.48 ± 0.13; 0.52 ± 0.09; p > 0.05). Finally, apical treatments with two different extract produced differential basolateral enterohormone secretions (basolateral PYY secretion vs control; animal extract, 0.35 ± 0.16; plant extract, 2.5 ± 0.74; p < 0.05). In conclusion, we report an ex vivo system called "Ap-to-Bas" for assaying vectorial transepithelial processes that makes it possible to work with several samples at the same time. It is an optimal device for enterohormone studies in the intestine.

Áreas temáticas: Zootecnia / recursos pesqueiros Saúde coletiva Química Planejamento urbano e regional / demografia Odontología Nutrição Medicina veterinaria Medicina ii Medicina i Materiais Interdisciplinar General medicine General chemistry General agricultural and biological sciences Food science & technology Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Educação Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, applied Chemistry (miscellaneous) Chemistry (all) Biotecnología Biodiversidade Astronomia / física Agriculture, multidisciplinary Agriculture Agricultural and biological sciences (miscellaneous) Agricultural and biological sciences (all)

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 00218561

Direcció de correo del autor: ximena.terra@urv.cat lluis.arola@urv.cat montserrat.pinent@urv.cat anna.ardevol@urv.cat

Identificador del autor: 0000-0003-1043-5844 0000-0003-2767-1974 0000-0003-3550-5378 0000-0003-0156-7538

Fecha de alta del registro: 2023-02-26

Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion

Enlace a la fuente original: https://pubs.acs.org/doi/10.1021/acs.jafc.8b03046

URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Journal Of Agricultural And Food Chemistry. 66 (44): 11622-11629

Referencia de l'ítem segons les normes APA: Ginés I, Gil-Cardoso K, Robles P, Arola L, Terra X, Blay M, Ardévol A, Pinent M. (2018). Novel ex Vivo Experimental Setup to Assay the Vectorial Transepithelial Enteroendocrine Secretions of Different Intestinal Segments.. Journal Of Agricultural And Food Chemistry, 66(44), 11622-11629. DOI: 10.1021/acs.jafc.8b03046

DOI del artículo: 10.1021/acs.jafc.8b03046

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2018

Tipo de publicación: Journal Publications