Articles producció científica> Química Analítica i Química Orgànica

In vitro validation of biomedical polyester-based scaffolds: Poly(lactide-co-glycolide) as model-case

  • Datos identificativos

    Identificador: imarina:5131947
    Autores:
    Gil-Castell O., Badia J.D., Ontoria-Oviedo I., Castellano D., Marco B., Rabal A., Bou J.J., Serra A., Monreal L., Blanes M., Sepúlveda P., Ribes-Greus A.
    Resumen:
    Monitoring and understanding the in vitro behaviour of polyester based scaffolds both comprising the study of the hydrolytic degradation and the cell seeding viability is essential to ensure the desired functionality, according to a given biomedical purpose. As a model case to compare the performance of techniques to monitor the in vitro behaviour, poly(lactide-co-glycolide) (PLGA) scaffolds were chosen. The in vitro hydrolytic degradation of PLGA scaffolds was carried out in water and phosphate buffered saline (PBS). The evolution of the mass loss, the molar mass, the thermal properties and the surface morphology were monitored. The hydrolytic degradation media was correspondingly evaluated by means of the study of the pH, the amount of acid released and the conductivity. In addition, the in vitro biocompatibility regarding the cell culture viability was studied under physiological conditions. The cellular adhesion, cellular ability to proliferate on the scaffold, the scaffold inflammatory profile and the effect of the scaffold degradation compounds on the cells were assessed. A comparative analysis of the exploited techniques in terms of promptness of identification, depth of knowledge, simplicity of obtaining results and cost of the technique was implemented. The results showed that, depending on the balance between the interest in ascertaining the trigger of degradation or deep into the knowledge of the causes and effects of cell culture viability, an appropriate plan of analysis of the validation of polyester-based scaffolds could be designed.
  • Otros:

    Autor según el artículo: Gil-Castell O., Badia J.D., Ontoria-Oviedo I., Castellano D., Marco B., Rabal A., Bou J.J., Serra A., Monreal L., Blanes M., Sepúlveda P., Ribes-Greus A.
    Departamento: Química Analítica i Química Orgànica
    Autor/es de la URV: Serra Albet, Maria Angels
    Palabras clave: Water-absorption Scaffold Release Polymer testing Polymer degradation Polylactide Polyester Poly(lactide-co-glycolide) (plga) Plga Performance In vitro validation Fibrous scaffolds Erosion Degradation behavior Biocomposites Biocompatibility Bastides
    Resumen: Monitoring and understanding the in vitro behaviour of polyester based scaffolds both comprising the study of the hydrolytic degradation and the cell seeding viability is essential to ensure the desired functionality, according to a given biomedical purpose. As a model case to compare the performance of techniques to monitor the in vitro behaviour, poly(lactide-co-glycolide) (PLGA) scaffolds were chosen. The in vitro hydrolytic degradation of PLGA scaffolds was carried out in water and phosphate buffered saline (PBS). The evolution of the mass loss, the molar mass, the thermal properties and the surface morphology were monitored. The hydrolytic degradation media was correspondingly evaluated by means of the study of the pH, the amount of acid released and the conductivity. In addition, the in vitro biocompatibility regarding the cell culture viability was studied under physiological conditions. The cellular adhesion, cellular ability to proliferate on the scaffold, the scaffold inflammatory profile and the effect of the scaffold degradation compounds on the cells were assessed. A comparative analysis of the exploited techniques in terms of promptness of identification, depth of knowledge, simplicity of obtaining results and cost of the technique was implemented. The results showed that, depending on the balance between the interest in ascertaining the trigger of degradation or deep into the knowledge of the causes and effects of cell culture viability, an appropriate plan of analysis of the validation of polyester-based scaffolds could be designed.
    Áreas temáticas: Química Polymers and plastics Polymer science Organic chemistry Odontología Medicina ii Medicina i Materials science, characterization & testing Materiais Interdisciplinar Engenharias iv Engenharias iii Engenharias ii Engenharias i Ciências biológicas iii Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Astronomia / física
    ISSN: 01429418
    Direcció de correo del autor: angels.serra@urv.cat
    Identificador del autor: 0000-0003-1387-0358
    Página final: 267
    Fecha de alta del registro: 2024-09-07
    Volumen de revista: 66
    Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
    Enlace a la fuente original: https://www.sciencedirect.com/science/article/abs/pii/S0142941817315647
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Polymer Testing. 66 256-267
    Referencia de l'ítem segons les normes APA: Gil-Castell O., Badia J.D., Ontoria-Oviedo I., Castellano D., Marco B., Rabal A., Bou J.J., Serra A., Monreal L., Blanes M., Sepúlveda P., Ribes-Greus (2018). In vitro validation of biomedical polyester-based scaffolds: Poly(lactide-co-glycolide) as model-case. Polymer Testing, 66(), 256-267. DOI: 10.1016/j.polymertesting.2018.01.027
    DOI del artículo: 10.1016/j.polymertesting.2018.01.027
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2018
    Página inicial: 256
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Materials Science, Characterization & Testing,Organic Chemistry,Polymer Science,Polymers and Plastics
    Water-absorption
    Scaffold
    Release
    Polymer testing
    Polymer degradation
    Polylactide
    Polyester
    Poly(lactide-co-glycolide) (plga)
    Plga
    Performance
    In vitro validation
    Fibrous scaffolds
    Erosion
    Degradation behavior
    Biocomposites
    Biocompatibility
    Bastides
    Química
    Polymers and plastics
    Polymer science
    Organic chemistry
    Odontología
    Medicina ii
    Medicina i
    Materials science, characterization & testing
    Materiais
    Interdisciplinar
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Engenharias i
    Ciências biológicas iii
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Biotecnología
    Astronomia / física
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