Articles producció científica> Bioquímica i Biotecnologia

The lipid composition of yeast cells modulates the response to iron deficiency

  • Datos identificativos

    Identificador: imarina:6232237
    Autores:
    Jordá TRomero AMPerea-García ARozès NPuig S
    Resumen:
    © 2020 Elsevier B.V. Iron is a vital micronutrient for all eukaryotes because it participates as a redox cofactor in multiple metabolic pathways, including lipid biosynthesis. In response to iron deficiency, the Saccharomyces cerevisiae iron-responsive transcription factor Aft1 accumulates in the nucleus and activates a set of genes that promote iron acquisition at the cell surface. In this study, we report that yeast cells lacking the transcription factor Mga2, which promotes the expression of the iron-dependent Δ9-fatty acid desaturase Ole1, display a defect in the activation of the iron regulon during the adaptation to iron limitation. Supplementation with exogenous unsaturated fatty acids (UFAs) or OLE1 expression rescues the iron regulon activation defect of mga2Δ cells. These observations and fatty acid measurements suggest that the mga2Δ defect in iron regulon expression is due to low UFA levels. Subcellular localization studies reveal that low UFAs cause a mislocalization of Aft1 protein to the vacuole upon iron deprivation that prevents its nuclear accumulation. These results indicate that Mga2 and Ole1 are essential to maintain the UFA levels required for Aft1-dependent activation of the iron regulon in response to iron deficiency, and directly connect the biosynthesis of fatty acids to the response to iron depletion.
  • Otros:

    Autor según el artículo: Jordá T; Romero AM; Perea-García A; Rozès N; Puig S
    Departamento: Bioquímica i Biotecnologia
    Autor/es de la URV: Rozès, Nicolas Andre Louis
    Palabras clave: Yeast Saccharomyces cerevisiae Ole1 Mga2 Iron deficiency Fatty acids Aft1 saccharomyces cerevisiae ole1 mga2 iron deficiency fatty acids aft1
    Resumen: © 2020 Elsevier B.V. Iron is a vital micronutrient for all eukaryotes because it participates as a redox cofactor in multiple metabolic pathways, including lipid biosynthesis. In response to iron deficiency, the Saccharomyces cerevisiae iron-responsive transcription factor Aft1 accumulates in the nucleus and activates a set of genes that promote iron acquisition at the cell surface. In this study, we report that yeast cells lacking the transcription factor Mga2, which promotes the expression of the iron-dependent Δ9-fatty acid desaturase Ole1, display a defect in the activation of the iron regulon during the adaptation to iron limitation. Supplementation with exogenous unsaturated fatty acids (UFAs) or OLE1 expression rescues the iron regulon activation defect of mga2Δ cells. These observations and fatty acid measurements suggest that the mga2Δ defect in iron regulon expression is due to low UFA levels. Subcellular localization studies reveal that low UFAs cause a mislocalization of Aft1 protein to the vacuole upon iron deprivation that prevents its nuclear accumulation. These results indicate that Mga2 and Ole1 are essential to maintain the UFA levels required for Aft1-dependent activation of the iron regulon in response to iron deficiency, and directly connect the biosynthesis of fatty acids to the response to iron depletion.
    Áreas temáticas: Química Molecular biology Medicina ii Medicina i Linguística e literatura Interdisciplinar Farmacia Engenharias iii Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Cell biology Biotecnología Biophysics Biodiversidade Biochemistry & molecular biology
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 1388-1981
    Direcció de correo del autor: nicolasrozes@urv.cat
    Identificador del autor: 0000-0001-9718-3429
    Fecha de alta del registro: 2023-02-22
    Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
    Enlace a la fuente original: https://www.sciencedirect.com/science/article/abs/pii/S1388198120300998?via%3Dihub
    Referencia al articulo segun fuente origial: Biochimica Et Biophysica Acta-Molecular And Cell Biology Of Lipids. 1865 (8): 158707-
    Referencia de l'ítem segons les normes APA: Jordá T; Romero AM; Perea-García A; Rozès N; Puig S (2020). The lipid composition of yeast cells modulates the response to iron deficiency. Biochimica Et Biophysica Acta-Molecular And Cell Biology Of Lipids, 1865(8), 158707-. DOI: 10.1016/j.bbalip.2020.158707
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI del artículo: 10.1016/j.bbalip.2020.158707
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2020
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Biochemistry & Molecular Biology,Biophysics,Cell Biology,Molecular Biology
    Yeast
    Saccharomyces cerevisiae
    Ole1
    Mga2
    Iron deficiency
    Fatty acids
    Aft1
    saccharomyces cerevisiae
    ole1
    mga2
    iron deficiency
    fatty acids
    aft1
    Química
    Molecular biology
    Medicina ii
    Medicina i
    Linguística e literatura
    Interdisciplinar
    Farmacia
    Engenharias iii
    Engenharias ii
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Cell biology
    Biotecnología
    Biophysics
    Biodiversidade
    Biochemistry & molecular biology
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