Autor según el artículo: Patraca, Ivan; Martinez, Nohora; Busquets, Oriol; Marti, Aleix; Pedros, Ignacio; Beas-Zarate, Carlos; Marin, Miguel; Ettcheto, Miren; Sureda, Francesc; Auladell, Carme; Camins, Antoni; Folch, Jaume
Departamento: Ciències Mèdiques Bàsiques Bioquímica i Biotecnologia
Autor/es de la URV: Folch Lopez, Jaume / Martí Zaragoza, Àlex / Martínez Micaelo, Nieves Beatriz / PATRACA FIERRO, JOSÉ IVÁN / Sureda Batlle, Francesc Xavier
Palabras clave: Tumor necrosis factor-alpha P38 mitogen-activated protein kinases P38 mapk Nitric oxide Neuroglia Mice, inbred c57bl Mice Map kinase signaling system Leptin Interleukin-1beta Interferon-gamma Inos Inflammation Glial cells Disease models, animal Cytokines Cytochrome-c Cells, cultured Cell survival Caspase-3 Apoptosis Anti-inflammatory agents Animals leptin inos glial cells cytochrome-c caspase-3
Resumen: © 2016 Institute of Pharmacology, Polish Academy of Sciences Background In the present work, we studied the modulatory effect of Leptin (Lep) against pro-inflammatory cytokines, tumour necrosis factor-alpha (TNFα), interleukin 1-beta (IL1β) and interferon-gamma (IFNγ), in primary glial cell cultures. Methods Glial cultures were treated with pro-inflammatory cytokines (TNFα, 20 ng/ml; IL1β, 20 ng/ml; IFNγ 20 ng/ml). Cells were pre-treated with Lep 500 nM, 1 h prior to cytokine treatment. NO released from glial cells was determined using the Griess reaction. Cell viability was determined by the MTT method. Protein expression was determined by western blot. Results Pre-treatment with 500 nM Lep produced an inhibitory effect on inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) production after glial cells exposure to pro-inflammatory cytokines. Anti-inflammatory effect can be related to a decrease in P38 MAP Kinase (MAPK) pathway activity. Treatment of glial cell cultures with Lep also reduced the intrinsic apoptotic pathway (cytochrome c release and caspase-3 activation). Conclusions We suggest that Lep would act as an anti-inflammatory factor in glial cells exposed to pro-inflammatory cytokines, exerting its function on p38 MAPK pathway and reducing NO production.
Áreas temáticas: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Pharmacology & pharmacy Pharmacology Odontología Nutrição Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Farmacia Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Biotecnología
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 17341140
Direcció de correo del autor: neus.martinez@urv.cat alex.marti@estudiants.urv.cat jaume.folch@urv.cat francesc.sureda@urv.cat
Identificador del autor: 0000-0002-5051-8858 0000-0002-7968-3929
Fecha de alta del registro: 2024-10-19
Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
Enlace a la fuente original: https://www.sciencedirect.com/science/article/abs/pii/S1734114016304273?via%3Dihub
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Pharmacological Reports. 69 (3): 409-418
Referencia de l'ítem segons les normes APA: Patraca, Ivan; Martinez, Nohora; Busquets, Oriol; Marti, Aleix; Pedros, Ignacio; Beas-Zarate, Carlos; Marin, Miguel; Ettcheto, Miren; Sureda, Francesc (2017). Anti-inflammatory role of Leptin in glial cells through p38 MAPK pathway inhibition. Pharmacological Reports, 69(3), 409-418. DOI: 10.1016/j.pharep.2016.12.005
DOI del artículo: 10.1016/j.pharep.2016.12.005
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2017
Tipo de publicación: Journal Publications