Autor según el artículo: Patraca I; Martínez N; Busquets O; Martí A; Pedrós I; Beas-Zarate C; Marin M; Ettcheto M; Sureda F; Auladell C; Camins A; Folch J

Departamento: Ciències Mèdiques Bàsiques Bioquímica i Biotecnologia

Autor/es de la URV: Folch Lopez, Jaume / PATRACA FIERRO, JOSÉ IVÁN / Sureda Batlle, Francesc Xavier

Palabras clave: P38 mapk Leptin Inos Glial cells Cytochrome-c Caspase-3 leptin inos glial cells cytochrome-c caspase-3

Resumen: © 2016 Institute of Pharmacology, Polish Academy of Sciences Background In the present work, we studied the modulatory effect of Leptin (Lep) against pro-inflammatory cytokines, tumour necrosis factor-alpha (TNFα), interleukin 1-beta (IL1β) and interferon-gamma (IFNγ), in primary glial cell cultures. Methods Glial cultures were treated with pro-inflammatory cytokines (TNFα, 20 ng/ml; IL1β, 20 ng/ml; IFNγ 20 ng/ml). Cells were pre-treated with Lep 500 nM, 1 h prior to cytokine treatment. NO released from glial cells was determined using the Griess reaction. Cell viability was determined by the MTT method. Protein expression was determined by western blot. Results Pre-treatment with 500 nM Lep produced an inhibitory effect on inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) production after glial cells exposure to pro-inflammatory cytokines. Anti-inflammatory effect can be related to a decrease in P38 MAP Kinase (MAPK) pathway activity. Treatment of glial cell cultures with Lep also reduced the intrinsic apoptotic pathway (cytochrome c release and caspase-3 activation). Conclusions We suggest that Lep would act as an anti-inflammatory factor in glial cells exposed to pro-inflammatory cytokines, exerting its function on p38 MAPK pathway and reducing NO production.

Áreas temáticas: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Pharmacology & pharmacy Pharmacology Odontología Nutrição Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Farmacia Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Biotecnología

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 17341140

Direcció de correo del autor: francesc.sureda@urv.cat jaume.folch@urv.cat

Identificador del autor: 0000-0002-7968-3929 0000-0002-5051-8858

Fecha de alta del registro: 2023-02-19

Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion

Enlace a la fuente original: https://www.sciencedirect.com/science/article/abs/pii/S1734114016304273?via%3Dihub

URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Pharmacological Reports. 69 (3): 409-418

Referencia de l'ítem segons les normes APA: Patraca I; Martínez N; Busquets O; Martí A; Pedrós I; Beas-Zarate C; Marin M; Ettcheto M; Sureda F; Auladell C; Camins A; Folch J (2017). Anti-inflammatory role of Leptin in glial cells through p38 MAPK pathway inhibition. Pharmacological Reports, 69(3), 409-418. DOI: 10.1016/j.pharep.2016.12.005

DOI del artículo: 10.1016/j.pharep.2016.12.005

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2017

Tipo de publicación: Journal Publications