Autor según el artículo: Gutiérrez-Martín, D; Marquès, M; Pons-Escoda, A; Vidal, N; Bruna, J; Restrepo-Montes, E; López-Serna, R; García-Sayago, F; Majos, C; Gago-Ferrero, P; Gil-Solsona, R
Departamento: Ciències Mèdiques Bàsiques
Autor/es de la URV: García Sayago, Francisco / Marquès Bueno, Montserrat
Palabras clave: Suspect screening Non-target screening Lc-hrms Human tissue Human biomonitoring Exposome Bead beating suspect screening non-target screening lc-hrms human biomonitoring bead beating
Resumen: Little is known about the presence of organic pollutants in human brain (and even less in brain tumors). In this regard, it is necessary to develop new analytical protocols capable of identifying a wide range of exogenous chemicals in this type of samples (by combining target, suspect and non-target strategies). These methodologies should be robust and simple. This is particularly challenging for solid samples, as reliable extraction and clean-up techniques should be combined to obtain an optimal result. Hence, the present study focuses on the development of an analytical methodology that allows the screening of a wide range of organic chemicals in brain and brain tumor samples. This protocol was based on a solid-liquid extraction based on bead beating, solid-phase extraction clean-up with multi-layer mixed-mode cartridges, reconstitution and LC–HRMS analysis. To evaluate the performance of the extraction methodology, a set of 66 chemicals (e.g., pharmaceuticals, biocides, or plasticizers, among others) with a wide range of physicochemical properties was employed. Quality control parameters (i.e., linear range, sensitivity, matrix effect (ME%), and recoveries (R%)) were calculated and satisfactory results were obtained for them (e.g., R% within 60–120% for 32 chemicals, or ME% higher than 50% (signal suppression) for 79% of the chemicals).
Áreas temáticas: Multidisciplinary sciences Multidisciplinary Medicina veterinaria Medical laboratory technology Interdisciplinar Engenharias iii Engenharias ii Clinical biochemistry Ciências biológicas iii Ciências ambientais Ciência de alimentos
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: francisco.garcia@urv.cat montserrat.marques@urv.cat
Identificador del autor: 0000-0003-0594-8370 0000-0001-6302-8578
Fecha de alta del registro: 2024-08-03
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://methods-x.com/article/S2215-0161(23)00072-9/fulltext
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Methodsx. 10 (102069):
Referencia de l'ítem segons les normes APA: Gutiérrez-Martín, D; Marquès, M; Pons-Escoda, A; Vidal, N; Bruna, J; Restrepo-Montes, E; López-Serna, R; García-Sayago, F; Majos, C; Gago-Ferrero, P; (2023). Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants. Methodsx, 10(102069), -. DOI: 10.1016/j.mex.2023.102069
DOI del artículo: 10.1016/j.mex.2023.102069
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2023
Tipo de publicación: Journal Publications