Articles producció científica> Ciències Mèdiques Bàsiques

Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants

  • Datos identificativos

    Identificador: imarina:9292125
    Autores:
    Gutiérrez-Martín, DMarquès, MPons-Escoda, AVidal, NBruna, JRestrepo-Montes, ELópez-Serna, RGarcía-Sayago, FMajos, CGago-Ferrero, PGil-Solsona, R
    Resumen:
    Little is known about the presence of organic pollutants in human brain (and even less in brain tumors). In this regard, it is necessary to develop new analytical protocols capable of identifying a wide range of exogenous chemicals in this type of samples (by combining target, suspect and non-target strategies). These methodologies should be robust and simple. This is particularly challenging for solid samples, as reliable extraction and clean-up techniques should be combined to obtain an optimal result. Hence, the present study focuses on the development of an analytical methodology that allows the screening of a wide range of organic chemicals in brain and brain tumor samples. This protocol was based on a solid-liquid extraction based on bead beating, solid-phase extraction clean-up with multi-layer mixed-mode cartridges, reconstitution and LC–HRMS analysis. To evaluate the performance of the extraction methodology, a set of 66 chemicals (e.g., pharmaceuticals, biocides, or plasticizers, among others) with a wide range of physicochemical properties was employed. Quality control parameters (i.e., linear range, sensitivity, matrix effect (ME%), and recoveries (R%)) were calculated and satisfactory results were obtained for them (e.g., R% within 60–120% for 32 chemicals, or ME% higher than 50% (signal suppression) for 79% of the chemicals).
  • Otros:

    Autor según el artículo: Gutiérrez-Martín, D; Marquès, M; Pons-Escoda, A; Vidal, N; Bruna, J; Restrepo-Montes, E; López-Serna, R; García-Sayago, F; Majos, C; Gago-Ferrero, P; Gil-Solsona, R
    Departamento: Ciències Mèdiques Bàsiques
    Autor/es de la URV: García Sayago, Francisco / Marquès Bueno, Montserrat
    Palabras clave: Suspect screening Non-target screening Lc-hrms Human tissue Human biomonitoring Exposome Bead beating suspect screening non-target screening lc-hrms human biomonitoring bead beating
    Resumen: Little is known about the presence of organic pollutants in human brain (and even less in brain tumors). In this regard, it is necessary to develop new analytical protocols capable of identifying a wide range of exogenous chemicals in this type of samples (by combining target, suspect and non-target strategies). These methodologies should be robust and simple. This is particularly challenging for solid samples, as reliable extraction and clean-up techniques should be combined to obtain an optimal result. Hence, the present study focuses on the development of an analytical methodology that allows the screening of a wide range of organic chemicals in brain and brain tumor samples. This protocol was based on a solid-liquid extraction based on bead beating, solid-phase extraction clean-up with multi-layer mixed-mode cartridges, reconstitution and LC–HRMS analysis. To evaluate the performance of the extraction methodology, a set of 66 chemicals (e.g., pharmaceuticals, biocides, or plasticizers, among others) with a wide range of physicochemical properties was employed. Quality control parameters (i.e., linear range, sensitivity, matrix effect (ME%), and recoveries (R%)) were calculated and satisfactory results were obtained for them (e.g., R% within 60–120% for 32 chemicals, or ME% higher than 50% (signal suppression) for 79% of the chemicals).
    Áreas temáticas: Multidisciplinary sciences Multidisciplinary Medicina veterinaria Medical laboratory technology Interdisciplinar Engenharias iii Engenharias ii Clinical biochemistry Ciências biológicas iii Ciências ambientais Ciência de alimentos
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: francisco.garcia@urv.cat montserrat.marques@urv.cat
    Identificador del autor: 0000-0003-0594-8370 0000-0001-6302-8578
    Fecha de alta del registro: 2024-08-03
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://methods-x.com/article/S2215-0161(23)00072-9/fulltext
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Methodsx. 10 (102069):
    Referencia de l'ítem segons les normes APA: Gutiérrez-Martín, D; Marquès, M; Pons-Escoda, A; Vidal, N; Bruna, J; Restrepo-Montes, E; López-Serna, R; García-Sayago, F; Majos, C; Gago-Ferrero, P; (2023). Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants. Methodsx, 10(102069), -. DOI: 10.1016/j.mex.2023.102069
    DOI del artículo: 10.1016/j.mex.2023.102069
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2023
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Clinical Biochemistry,Medical Laboratory Technology,Multidisciplinary Sciences
    Suspect screening
    Non-target screening
    Lc-hrms
    Human tissue
    Human biomonitoring
    Exposome
    Bead beating
    suspect screening
    non-target screening
    lc-hrms
    human biomonitoring
    bead beating
    Multidisciplinary sciences
    Multidisciplinary
    Medicina veterinaria
    Medical laboratory technology
    Interdisciplinar
    Engenharias iii
    Engenharias ii
    Clinical biochemistry
    Ciências biológicas iii
    Ciências ambientais
    Ciência de alimentos
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