Conjunts de dades de producció científicaBioquímica i Biotecnologia

RT-qPCR dCT values of genes analyzed in colon

  • Datos identificativos

    Identificador:  PC:4131
    Autores:  Canela, Nuria
    Resumen:
    RT-qPCR dCt values of gene expression from colon, small intestine and liver tissues from germ free mice monocolonized with E. coli expression bile salt hydrolase activity. Mice monocolonized with non-BSH expressing E. coli, non-monocolonized and conventionally-raised mice were used as controls, and RT-qpCR dCT values of colon organoids incubated with a mix of bile acids, LPS or a mix of bile acids + LPS. Values are normalized to the expression levels of ß-actin
  • Otros:

    Tipo de documento: info:eu-repo/semantics/other
    DOI: 10.5281/zenodo.6598104
    Publicaciones relacionadas: Núñez-Sánchez, M. A., Herisson, F. M., Keane, J. M., García-González, N., Rossini, V., Pinhiero, J., Daly, J., Bustamante-Garrido, M., Hueston, C. M., Patel, S., Canela, N., Herrero, P., Claesson, M. J., Melgar, S., Nally, K., Caplice, N. M., & Gahan, C. G. M. (2022). Microbial bile salt hydrolase activity influences gene expression profiles and gastrointestinal maturation in infant mice. Gut Microbes, 14(1), 2149023. https://doi.org/10.1080/19490976.2022.2149023
    Departamento: Bioquímica i Biotecnologia
    Autor: Canela, Nuria
    Fecha alta repositorio: 2022-05-31
    Año de publicación de la dataset: 2022
    Materia: Bioquímica
    Identificador del investigador: 0000-0003-0261-2396
    DOI de la publicación relacionada: 10.1080/19490976.2022.2149023
    Idioma: en
    Publicado por (editorial): Universitat Rovira i Virgili (URV)
    Derechos de acceso: info:eu-repo/semantics/openAccess
    Resumen: RT-qPCR dCt values of gene expression from colon, small intestine and liver tissues from germ free mice monocolonized with E. coli expression bile salt hydrolase activity. Mice monocolonized with non-BSH expressing E. coli, non-monocolonized and conventionally-raised mice were used as controls, and RT-qpCR dCT values of colon organoids incubated with a mix of bile acids, LPS or a mix of bile acids + LPS. Values are normalized to the expression levels of ß-actin
  • Palabras clave:

    Bile
    organoid
    colon
    neonatal
    infant
    gut development
    intestine
    stem cell
    Bioquímica
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