Estudio bioquímico y molecular de la producción de precursores de carbamato de etilo por bacteria lácticas asociadas al proceso de vinificación

Identificador:  TDX:475

Handle: https://hdl.handle.net/20.500.11797/TDX475

Autores:  Araque Granados, Maria Isabel

Resumen:
The most frequently isolated lactic acid bacteria (LAB) of wine were screened for arginine degradation. The generation of precursors such as citrulline and carbamyl phosphate for generation ethyl carbamate (CE) was review. Degrading strains included all heterofermentative lactobacilli, Oenococcus oeni, Pediococcus pentosaceus and some strains of Leuconostoc mesenteroides and Lactobacillus plantarum. The presence and the expression of operon arc genes for arginine-deiminase pathway was studied in several strains of different species of LAB. In some strains, there no existence of all genes involved because theirs aren't always grouped together as an operon. In other strains, the pathway isn't active or kinetic activity of each encoded enzymes is different. The environmental condition of growth can affect in the rate of degradation which can lead to accumulation of precursors of CE at media.