Development and validation of an analytical method: quantification of total aflatoxin in peach purées using the ELISA method

Identifier:  TFG:10076

Handle: https://hdl.handle.net/20.500.11797/TFG10076

Authors:  Giraut Abad, Joel Felip

Abstract:
An analytical method for the quantification of total aflatoxin in peach purees has been developed and validated, using immunoaffinity columns and the ELISA (enzyme-linked immunosorbent assay) technique. The study includes the evaluation of different extraction solvents, eluent volumes and the use of the surfactant Tween 20 to improve the recovery of the analyte. As a result, the extraction conditions have been optimized to achieve a recovery of 70%, in accordance with the Codex Alimentarius. Finally, the developed method has been statistically validated in terms of recovery, precision, uncertainty and LOQ, showing itself to be suitable for the determination of total aflatoxin in this food matrix.