Study of the interaction of muscular circadian rhythms with the therapeutic effect of a grape-seed proanthocyanidin extract in rats with metabolic syndrome

Identifier:  TFG:3006

Handle: https://hdl.handle.net/20.500.11797/TFG3006

Authors:  Royo García, Alba

Abstract:
The metabolic syndrome (MetS) is a group of cardiometabolic factors that contribute to the development of type II diabetes and cardiovascular diseases. The rising prevalence and the risk associated with it, releases an important global alarm concerning public health. Previous studies have associated how high fat diet (HFD) can affect mitochondrial dynamics and circadian rhythms. However, there have been found therapeutic benefits with polyphenols as a possible treatment, one of these being a grape seed proanthocyanidin extract (GSPE).This dissertation is focus on the impact that GSPE has on circadian muscular rhythms in a MetS model. This way, the state hypothesis consists in the existence of a circadian swift in energy metabolism as well as mitochondrial dynamics of skeletal muscle due to HFD. In order to prove this hypothesis, biochemical parameters, circadian muscular clock genes, mitochondrial dynamics genes and proteins and metabolic proteins are studied in an experimental MetS model. Two Fischer 344 rats were feed for 9 weeks with standard or cafeteria diet (CAF) and supplemented with GSPE or vehicle in the last 4 weeks. CAF rats are considered a robust MetS model, which is evidenced by subsequent biochemical analysis. Our results have proven this model due to the body weight gain, food intake and hyperglycemia. Same way, GSPE shows a beneficial effect in the glycemia levels at 9PM. However, soleus muscle shows less sensitivity than other organs regarding molecular clock disruption by HFD. To sum up, GSPE has shown beneficial effects modulating metabolic disorders, for this reason, it is considered as a good candidate as a therapeutic strategy for MetS patients.