Impacto de la deleción del gen PYK2 en el metabolismo de S. Cervisiae Lalvin Clos en condiciones fermentativas y respiratorias

Creation date in repository: 2026/01/22

Abstract: En este trabajo se analizó el efecto de la deleción del gen PYK2 en Saccharomyces cerevisiae Lalvin Clos para evaluar su influencia en el crecimiento y la adaptación metabólica frente a distintas fuentes de carbono. Se compararon parámetros de crecimiento, consumo de sustratos, producción de metabolitos y expresión de genes clave. La cepa Δpyk2 creció en condiciones fermentativas, pero no en fuentes respiratorias ni reutilizó etanol, a diferencia de la parental. Una mutación mitocondrial inesperada limitó la interpretación de los resultados. El estudio aporta una base para futuras investigaciones sobre el papel específico de PYK2 en el metabolismo de S. cerevisiae.; In this work, we characterized the effect of the PYK2 gene deletion in the wine strain Saccharomyces cerevisiae Lalvin Clos, with the aim of evaluating its influence on growth and metabolic adaptation to different fermentable and non-fermentable carbon sources. Growth parameters, substrate consumption, and metabolite production were compared between the Δpyk2 mutant strain and the control strain, and the expression of key genes involved in fermentative and respiratory pathways, such as CAT8, PDC2, and CYC1, was also analyzed. The Δpyk2 strain showed similar growth to the Lalvin Clos strain in media with fermentable carbon sources, but was unable to grow on nonfermentable sources or to reuse the ethanol generated after fermentation for the diauxic growth observed in the parental strain. In contrast, when compared with a laboratory strain, both the parental strain and its Δpyk2 mutant were able to grow on non-fermentable sources and consume ethanol. During the experimental process, the presence of a mitochondrial mutation was detected in the mutant strain. Unlike the PYK2 deletion, which according to the literature does not affect growth in different carbon sources, it does cause a significant change in growth, especially in media with respiratory sources. The presence of this unexpected mutation limits the interpretation of the results obtained. Therefore, although differences in metabolic and adaptive capacity were observed between the PYK2 mutant and the parental strain, it was not possible to establish a conclusive causal relationship between these differences and the loss of PYK2 function. This study lays the groundwork for future research to more precisely isolate and analyze the specific role of PYK2 in S. cerevisiae metabolism, especially in its interaction with the glycolytic complex and melatonin, relevant elements in cellular metabolic regulation.; En aquest treball es va analitzar l'efecte de la deleció del gen PYK2 a Saccharomyces cerevisiae Lalvin Clos per avaluar-ne la influència en el creixement i l'adaptació metabòlica davant de diferents fonts de carboni. Es van comparar paràmetres de creixement, consum de substrats, producció de metabòlits i expressió de gens clau. El cep Δpyk2 va créixer en condicions fermentatives, però no en fonts respiratòries ni va reutilitzar etanol, a diferència de la parental. Una mutació mitocondrial inesperada va limitar la interpretació dels resultats. L'estudi aporta una base per a futures investigacions sobre el paper específic de PYK2 al metabolisme de S. cerevisiae.

Subject: Fermentació

Language: es

Subject areas: Bioquímica y biotecnología; Biochemistry and biotechnology; Bioquímica i biotecnologia

Department: Bioquímica i Biotecnologia

Student: García García, Aida

Academic year: 2024-2025

Title in different languages: Impact of Pyk2 gene deletion on the metabolism of S. Cerevisiae Lalvin Clos under fermentative and respiratory conditions; Impacte de la deleció del gen PYK2 en el metabolisme de S. Cerevisiae Lalvin Clos en condiciones fermentatives i respiratòries

Work's public defense date: 2025-06-10

Access rights: info:eu-repo/semantics/openAccess

Keywords: S. cerevisiae, PYK2, metabolismo del carbono; S. cerevisiae, PYK2, carbon metabolism; S. cerevisiae, PYK2, metabolisme del carboni

Confidenciality: No

TFG credits: 12

Title in original language: Impacto de la deleción del gen PYK2 en el metabolismo de S. Cervisiae Lalvin Clos en condiciones fermentativas y respiratorias

Project director: Torija Martínez, María Jesús

Education area(s): Biotecnologia

Entity: Universitat Rovira i Virgili (URV)