Screening of synthetic dual vector systems for monitoring e3 ligase activity in plant protoplasts

Identifier:  TFG:9342

Handle: https://hdl.handle.net/20.500.11797/TFG9342

Authors:  Arjona Martí, Aida

Abstract:
Protein homeostasis depends on continuous turnover through synthesis and degradation. The ubiquitin-proteasome system (UPS) is the main pathway for degradation, where proteins are tagged with ubiquitin and directed to the 26S proteasome. E3 ubiquitin ligases are crucial for recognizing substrates and attaching ubiquitin, regulating processes such as the cell cycle, DNA repair, and hormone signaling. However, assessing the effects of ubiquitination is difficult, as monitoring often relies on detectable reporter systems. To address this, we developed a synthetic dual-vector reporter in Arabidopsis thaliana protoplasts. Although initial results were inconclusive, this strategy shows promise for studying E3 ligase activity.