Entity: Universitat Rovira i Virgili (URV)
Confidenciality: No
Education area(s): Begudes Fermentades
Title in different languages: Isolation, identification and characterization of indigenous yeasts
Abstract: The selection of autochthonous yeasts for the production of wine can be used to enhance the differential oenological characteristics that determine a wine-growing region. In this work, autochthonous yeasts have been isolated, identified and characterized, based on the Albariño grape variety of D.O. Rías Baixas and during spontaneous fermentations of white wines from the same variety. We evaluated fermentative abilities and characteristics of S. cerevisiae isolated strains by fermentation with pure cultures and massive inoculation at laboratory-scale. Subsequently, fermentations were made with mixed cultures of S. cerevisiae and non- Saccharomyces. For the selection of yeast strains, different oenological (production of ethanol, volatile acidity, glycerol, pH, etc.) and microbiological parameters (evolution and ability to impose during alcoholic fermentation) were considered. A total of eighteen strains of S. cerevisiae, typified by Delta technique, have been obtained, and nine non-Saccharomyces yeasts species have been identified using RFLP-PCR of the 5,8S-ITS region (Rhodotorula glutinis, Metschnikowia pulcherrima, Cryptococcus magnus, Cryptococcus flavescens, Hanseniaspora uvarum, Hanseniaspora guilliermondii, Torulaspora delbrueckii, Pichia membranaefaciens and Candida zemplinina). The selected S. cerevisiae strains except two showed optimal fermentative aptitudes for the production of wines that reflect the characteristics of the terroir and the grape variety and that differ from the rest. However, future work is needed to define the oenological characteristics of selected yeasts, both in pure inoculations of S. cerevisiae and in mixed inoculations with non-Saccharomyces.
Subject: Enologia
Academic year: 2017-2018
Language: Català
Work's public defense date: 2018-06-21
Subject areas: Enology
Student: Batle Soler, Laura
Work's codirector: Batle, Laura; Miró, Gemma; Bargalló, Marta; Mislata, Ana María; Puxeu, Miquel; Andorrà, Imma
Department: Bioquímica i Biotecnologia
Creation date in repository: 2018-10-24
TFM credits: 15
Keywords: indigenous yeasts, S. cerevisiae, oenological characterization
Title in original language: Selecció i caracterització de llevats autòctons
Project director: Andorrà Solsona, Imma
Repositori URV